Biological process for coproduction of hydrogen and thermophilic enzymes during CO fermentation SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Lee, Seong Hyuk | - |
dc.contributor.author | Lee, Sung-Mok | - |
dc.contributor.author | Lee, Jung-Hyun | - |
dc.contributor.author | Lee, Hyun Sook | - |
dc.contributor.author | Kang, Sung Gyun | - |
dc.date.accessioned | 2020-12-10T07:49:24Z | - |
dc.date.available | 2020-12-10T07:49:24Z | - |
dc.date.created | 2020-05-08 | - |
dc.date.issued | 2020-06 | - |
dc.identifier.issn | 0960-8524 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/38643 | - |
dc.description.abstract | To develop a thermophilic cell factory system that uses CO gas, we attempted to engineer a hyperthermophilic carboxydotrophic hydrogenic archaeon Thermococcus onnurineus NA1 to be capable of producing thermophilic enzymes along with hydrogen (H-2). The mutant strains 156T-AM and 156T-POL were constructed to have another copy of a gene encoding alpha-amylase or DNA polymerase, respectively, and exhibited growth rates and H-2 production rates distinct from those of the parental strain, 156T, in gas fermentation using 100% CO or coal-gasified syngas. Purified alpha-amylase displayed starch-hydrolyzing activity, and whole-cell extracts of 156T-AM showed saccharifying activity for potato peel waste. PCR amplification was used to demonstrate that purified DNA polymerase was free from bacterial DNA contamination, in contrast to commercial bacteria-made enzymes. This study demonstrated that this archaeal strain could coproduce enzymes and H-2 using CO-containing gas, providing a basis for cell factories to upcycle industrial waste gas. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | DEPENDENT H-2 PRODUCTION | - |
dc.subject | HYPERTHERMOPHILIC ARCHAEON | - |
dc.subject | DNA-POLYMERASE | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | BACTERIAL-DNA | - |
dc.subject | SYNTHESIS GAS | - |
dc.subject | POTATO PEEL | - |
dc.subject | SEQUENCE | - |
dc.subject | DENSITY | - |
dc.subject | CLONING | - |
dc.title | Biological process for coproduction of hydrogen and thermophilic enzymes during CO fermentation | - |
dc.type | Article | - |
dc.citation.title | BIORESOURCE TECHNOLOGY | - |
dc.citation.volume | 305 | - |
dc.contributor.alternativeName | 이성혁 | - |
dc.contributor.alternativeName | 이성목 | - |
dc.contributor.alternativeName | 이정현 | - |
dc.contributor.alternativeName | 이현숙 | - |
dc.contributor.alternativeName | 강성균 | - |
dc.identifier.bibliographicCitation | BIORESOURCE TECHNOLOGY, v.305 | - |
dc.identifier.doi | 10.1016/j.biortech.2020.123067 | - |
dc.identifier.scopusid | 2-s2.0-85080055778 | - |
dc.identifier.wosid | 000522033100004 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.subject.keywordPlus | DEPENDENT H-2 PRODUCTION | - |
dc.subject.keywordPlus | HYPERTHERMOPHILIC ARCHAEON | - |
dc.subject.keywordPlus | DNA-POLYMERASE | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | BACTERIAL-DNA | - |
dc.subject.keywordPlus | SYNTHESIS GAS | - |
dc.subject.keywordPlus | POTATO PEEL | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | DENSITY | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordAuthor | Cell factory | - |
dc.subject.keywordAuthor | Thermococcus onnurineus NA1 | - |
dc.subject.keywordAuthor | Coproduction | - |
dc.subject.keywordAuthor | Hydrogen | - |
dc.subject.keywordAuthor | Thermophilic enzyme | - |
dc.relation.journalWebOfScienceCategory | Agricultural Engineering | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Energy & Fuels | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Energy & Fuels | - |