Biological process for coproduction of hydrogen and thermophilic enzymes during CO fermentation SCIE SCOPUS

Cited 2 time in WEB OF SCIENCE Cited 2 time in Scopus
Title
Biological process for coproduction of hydrogen and thermophilic enzymes during CO fermentation
Author(s)
Lee, Seong Hyuk; Lee, Sung-Mok; Lee, Jung-Hyun; Lee, Hyun Sook; Kang, Sung Gyun
KIOST Author(s)
Lee, Seong Hyuk(이성혁)Lee, Jung Hyun(이정현)Lee, Hyun Sook(이현숙)Kang, Sung Gyun(강성균)
Publication Year
2020-06
Abstract
To develop a thermophilic cell factory system that uses CO gas, we attempted to engineer a hyperthermophilic carboxydotrophic hydrogenic archaeon Thermococcus onnurineus NA1 to be capable of producing thermophilic enzymes along with hydrogen (H-2). The mutant strains 156T-AM and 156T-POL were constructed to have another copy of a gene encoding alpha-amylase or DNA polymerase, respectively, and exhibited growth rates and H-2 production rates distinct from those of the parental strain, 156T, in gas fermentation using 100% CO or coal-gasified syngas. Purified alpha-amylase displayed starch-hydrolyzing activity, and whole-cell extracts of 156T-AM showed saccharifying activity for potato peel waste. PCR amplification was used to demonstrate that purified DNA polymerase was free from bacterial DNA contamination, in contrast to commercial bacteria-made enzymes. This study demonstrated that this archaeal strain could coproduce enzymes and H-2 using CO-containing gas, providing a basis for cell factories to upcycle industrial waste gas.
ISSN
0960-8524
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/38643
DOI
10.1016/j.biortech.2020.123067
Bibliographic Citation
BIORESOURCE TECHNOLOGY, v.305, 2020
Publisher
ELSEVIER SCI LTD
Subject
DEPENDENT H-2 PRODUCTION; HYPERTHERMOPHILIC ARCHAEON; DNA-POLYMERASE; ESCHERICHIA-COLI; BACTERIAL-DNA; SYNTHESIS GAS; POTATO PEEL; SEQUENCE; DENSITY; CLONING
Keywords
Cell factory; Thermococcus onnurineus NA1; Coproduction; Hydrogen; Thermophilic enzyme
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER SCI LTD
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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