Transcriptional analysis of disk abalone (Haliotis discus discus) antioxidant enzymes against marine bacteria and virus challenge SCIE SCOPUS

Cited 16 time in WEB OF SCIENCE Cited 17 time in Scopus
Title
Transcriptional analysis of disk abalone (Haliotis discus discus) antioxidant enzymes against marine bacteria and virus challenge
Author(s)
De Zoysa, Mahanama; Whang, Ilson; Nikapitiya, Chamilani; Oh, Chulhong; Choi, Cheol Young; Lee, Jehee
KIOST Author(s)
Oh, Chul Hong(오철홍)
Publication Year
2011-07
Abstract
Diverse antioxidant enzymes are essential for marine organisms to overcome oxidative stress as well as for the fine-tuning of immune reactions through activating different signal transduction pathways. This study describes the transcriptional analysis of antioxidant enzymes of disk abalone by challenging with bacteria (Vibrio alginolyticus, Vibrio parahemolyticus, and Listeria monocytogenes) and viral hemorrhagic septicemia virus (VHSV). Upon bacteria and VHSV challenge, Manganese superoxide dismutase (MnSOD), Copper, Zinc superoxide dismutase (CuZnSOD), catalase, thioredoxin peroxidase (TPx), Selenium-dependent glutathione peroxidase (SeGPx), and thioredoxin-2 (TRx-2) expression levels were altered in gills, and hemocytes at different magnitudes. In gills, only MnSOD, catalase, and SeGPx genes were completely upregulated by post-challenge of bacterial and VHSV. Among them, SeGPx demonstrated strong upregulation by 16-fold (bacteria) and 2-fold (VHSV) in gills, and 5-fold (bacteria) and 3.0-fold (VHSV) in hemocytes. None of the genes examined were downregulated (in gills and hemocytes) by bacteria challenge even though CuZnSOD and TPx showed downregulation (completely) in hemocytes by VHSV. In general, abalone hemocytes had lower potential to induce antioxidant enzyme transcripts upon bacteria and VHSV challenge than gills. Based upon these results, we suggest that abalones induce oxidative stress in tissues during the bacteria and VHSV challenge, and the identified response of antioxidant enzymes could be supported for maintaining a low-level of reactive oxygen species (ROS) that may serve as a signal for activating immune reactions against pathogenic conditions. (C) 2011 Elsevier Ltd. All rights reserved.
ISSN
1050-4648
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3849
DOI
10.1016/j.fsi.2011.04.001
Bibliographic Citation
FISH & SHELLFISH IMMUNOLOGY, v.31, no.1, pp.155 - 160, 2011
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Subject
DIVERSICOLOR-SUPERTEXTA; VIBRIO-PARAHAEMOLYTICUS; DEFENSE-MECHANISMS; OXIDATIVE STRESS; IMMUNE-RESPONSES; GENE-EXPRESSION; LEUKOCYTES; HEMOCYTES; INFECTION; PARASITE
Keywords
Antioxidant enzymes; Disk abalone (Haliotis discus discus); ROS; Bacteria; VHSV
Type
Article
Language
English
Document Type
Article
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Related Researcher
Research Interests

Recombinant protein,Marine microorganisms,Functional proteins,재조합단백질,해양미생물,기능성단백질

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