Enrichment and proteome analysis of a hyperthermostable protein set of archaeon Thermococcus onnurineus NA1 SCIE SCOPUS

Cited 8 time in WEB OF SCIENCE Cited 8 time in Scopus
Title
Enrichment and proteome analysis of a hyperthermostable protein set of archaeon Thermococcus onnurineus NA1
Author(s)
Yun, Sung-Ho; Choi, Chi-Won; Kwon, Sang Oh; Lee, Yeol Gyun; Chung, Young-Ho; Jung, Hoi Jong; Kim, Yun-Jae; Lee, Jung-Hyun; Choi, Jong-Soon; Kim, Soohyun; Kim, Seung Il
KIOST Author(s)
Lee, Jung Hyun(이정현)
Publication Year
2011-07
Abstract
Thermococcus onnurineus NA1 is a hyperthermophilic archaeon that can be used for the screening of thermophilic enzymes. Previously, we characterized the metabolic enzymes of the cytosolic proteome by two-dimensional electrophoresis/tandem mass spectrometry (2-DE/MS-MS). In this study, we identified a subset of hyperthermostable proteins in the cytosolic proteome using enrichment by in vitro heat treatment and protein identification. After heat treatment at 100A degrees C for 2 h, 13 and 149 proteins were identified from the soluble proteome subset by 2-DE/MS-MS and 1-DE/MS-MS analysis, respectively. Representative proteins included intracellular protease I, thioredoxin reductase, triosephosphate isomerase, putative hydroperoxide reductase, proteasome, and translation initiation factors. Intracellular protease, deblocking aminopeptidases, and fructose-1,6-bisphosphatase were overexpressed in Escherichia coli and biological activity above 85A degrees C was confirmed. The folding transition temperature (Tm) of identified proteins was analyzed using the in silico prediction program TargetStar. The proteins enriched with the heat treatment have higher Tm than the homologous proteins from mesophilic strains. These results suggested that the heat-stable protein set of hyperthermophilic T. onnurineus NA1 can be effectively fractionated and enriched by in vitro heat treatment.
ISSN
1431-0651
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3848
DOI
10.1007/s00792-011-0376-1
Bibliographic Citation
EXTREMOPHILES, v.15, no.4, pp.451 - 461, 2011
Publisher
SPRINGER JAPAN KK
Subject
COMPLETE GENOME SEQUENCE; HYPERTHERMOPHILIC ARCHAEON; THERMOANAEROBACTER-TENGCONGENSIS; BIOCHEMICAL-CHARACTERIZATION; PYROCOCCUS-FURIOSUS; FRUCTOSE-1,6-BISPHOSPHATASE; THERMOSTABILITY; CRENARCHAEON; PURIFICATION
Keywords
Archaea; Thermococcus; Hyperthermostable; Proteome
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER JAPAN KK
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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