Chromene induces apoptosis via caspase-3 activation in human leukemia HL-60 cells
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Title
- Chromene induces apoptosis via caspase-3 activation in human leukemia HL-60 cells
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Author(s)
- Heo, Soo-Jin; Kim, Kil-Nam; Yoon, Weon-Jong; Oh, Chulhong; Choi, Young-Ung; Affan, Abu; Lee, Yeon-Ju; Lee, Hyi-Seung; Kang, Do-Hyung
- KIOST Author(s)
- Heo, Soo Jin(허수진); Oh, Chul Hong(오철홍); Choi, Young Ung(최영웅); Lee, Yeon Ju(이연주); Lee, Hyi Seung(이희승); Kang, Do Hyung(강도형)
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Alternative Author(s)
- 허수진; 오철홍; 최영웅; Abu Affan; 이연주; 이희승; 강도형
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Publication Year
- 2011-09
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Abstract
- In this study, the potent anti-tumor effects of brown algae on human leukemia HL-60 cells were investigated. The Sargassum siliquastrum extract among the 14 species of brown algae exhibited profound growth inhibitory effect on HL-60 cells in the 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide (MTT) assay, therefore, S. siliquastrum was selected for use in further experiments. The highest inhibitory activity of S. siliquastrum on HL-60 cells was detected in the chloroform fraction, and the active compound was identified as a kind of chromene, sargachromanol E (SE). SE treatment showed significant growth inhibitory effects on HL-60 cells in a dose-dependent manner by inducing apoptosis, as evidenced by the formation of apoptotic bodies, fragmented DNA ladder, and the accumulation of DNA in the sub-G(1) phase of cell cycle. SE induced apoptosis was accompanied by downregulation of Bcl-xL, upregulation of Bax, activation of caspase-3, and cleavage of poly (ADP-ribose) polymerase (PARP). Moreover, z-DEVD-fmk, a caspase-3 inhibitor. significantly inhibited cell cytotoxicity, apoptotic characteristics such as apoptotic bodies, sub-G(1) DNA content, and cleavage of PARP induced by SE. These results suggest that SE exerts its growth inhibitory effects on HL-60 cells through caspase-3-mediated induction of apoptosis. Therefore, SE offers promising chemotherapeuric potential to prevent cancers such as human leukemia. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.
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ISSN
- 0278-6915
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/3825
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DOI
- 10.1016/j.fct.2011.05.011
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Bibliographic Citation
- FOOD AND CHEMICAL TOXICOLOGY, v.49, no.9, pp.1998 - 2004, 2011
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Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
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Subject
- CANCER-CHEMOTHERAPY; NATURAL-PRODUCTS; BCL-2; MITOCHONDRIA; INDUCTION; FAMILY; PLASTOQUINONES; PATHWAY
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Keywords
- Sargassum siliquastrum; Chromene; Sargachromanol E; Apoptosis; Caspase-3
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Type
- Article
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Language
- English
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Document Type
- Article
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