Chromene induces apoptosis via caspase-3 activation in human leukemia HL-60 cells SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Heo, Soo-Jin | - |
dc.contributor.author | Kim, Kil-Nam | - |
dc.contributor.author | Yoon, Weon-Jong | - |
dc.contributor.author | Oh, Chulhong | - |
dc.contributor.author | Choi, Young-Ung | - |
dc.contributor.author | Affan, Abu | - |
dc.contributor.author | Lee, Yeon-Ju | - |
dc.contributor.author | Lee, Hyi-Seung | - |
dc.contributor.author | Kang, Do-Hyung | - |
dc.date.accessioned | 2020-04-20T07:43:30Z | - |
dc.date.available | 2020-04-20T07:43:30Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2011-09 | - |
dc.identifier.issn | 0278-6915 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/3825 | - |
dc.description.abstract | In this study, the potent anti-tumor effects of brown algae on human leukemia HL-60 cells were investigated. The Sargassum siliquastrum extract among the 14 species of brown algae exhibited profound growth inhibitory effect on HL-60 cells in the 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide (MTT) assay, therefore, S. siliquastrum was selected for use in further experiments. The highest inhibitory activity of S. siliquastrum on HL-60 cells was detected in the chloroform fraction, and the active compound was identified as a kind of chromene, sargachromanol E (SE). SE treatment showed significant growth inhibitory effects on HL-60 cells in a dose-dependent manner by inducing apoptosis, as evidenced by the formation of apoptotic bodies, fragmented DNA ladder, and the accumulation of DNA in the sub-G(1) phase of cell cycle. SE induced apoptosis was accompanied by downregulation of Bcl-xL, upregulation of Bax, activation of caspase-3, and cleavage of poly (ADP-ribose) polymerase (PARP). Moreover, z-DEVD-fmk, a caspase-3 inhibitor. significantly inhibited cell cytotoxicity, apoptotic characteristics such as apoptotic bodies, sub-G(1) DNA content, and cleavage of PARP induced by SE. These results suggest that SE exerts its growth inhibitory effects on HL-60 cells through caspase-3-mediated induction of apoptosis. Therefore, SE offers promising chemotherapeuric potential to prevent cancers such as human leukemia. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.subject | CANCER-CHEMOTHERAPY | - |
dc.subject | NATURAL-PRODUCTS | - |
dc.subject | BCL-2 | - |
dc.subject | MITOCHONDRIA | - |
dc.subject | INDUCTION | - |
dc.subject | FAMILY | - |
dc.subject | PLASTOQUINONES | - |
dc.subject | PATHWAY | - |
dc.title | Chromene induces apoptosis via caspase-3 activation in human leukemia HL-60 cells | - |
dc.type | Article | - |
dc.citation.endPage | 2004 | - |
dc.citation.startPage | 1998 | - |
dc.citation.title | FOOD AND CHEMICAL TOXICOLOGY | - |
dc.citation.volume | 49 | - |
dc.citation.number | 9 | - |
dc.contributor.alternativeName | 허수진 | - |
dc.contributor.alternativeName | 오철홍 | - |
dc.contributor.alternativeName | 최영웅 | - |
dc.contributor.alternativeName | Abu Affan | - |
dc.contributor.alternativeName | 이연주 | - |
dc.contributor.alternativeName | 이희승 | - |
dc.contributor.alternativeName | 강도형 | - |
dc.identifier.bibliographicCitation | FOOD AND CHEMICAL TOXICOLOGY, v.49, no.9, pp.1998 - 2004 | - |
dc.identifier.doi | 10.1016/j.fct.2011.05.011 | - |
dc.identifier.scopusid | 2-s2.0-79960843445 | - |
dc.identifier.wosid | 000295188600014 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | CANCER-CHEMOTHERAPY | - |
dc.subject.keywordPlus | NATURAL-PRODUCTS | - |
dc.subject.keywordPlus | BCL-2 | - |
dc.subject.keywordPlus | MITOCHONDRIA | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | FAMILY | - |
dc.subject.keywordPlus | PLASTOQUINONES | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordAuthor | Sargassum siliquastrum | - |
dc.subject.keywordAuthor | Chromene | - |
dc.subject.keywordAuthor | Sargachromanol E | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Caspase-3 | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalResearchArea | Toxicology | - |