Proteome analysis of Thermococcus onnurineus NA1 reveals the expression of hydrogen gene cluster under carboxydotrophic growth SCIE SCOPUS

Cited 11 time in WEB OF SCIENCE Cited 12 time in Scopus
Title
Proteome analysis of Thermococcus onnurineus NA1 reveals the expression of hydrogen gene cluster under carboxydotrophic growth
Author(s)
Yun, Sung-Ho; Kwon, Sang Oh; Park, Gun Wook; Kim, Jin Young; Kang, Sung Gyun; Lee, Jung-Hyun; Chung, Young-Ho; Kim, Soohyun; Choi, Jong-Soon; Kim, Seung Il
KIOST Author(s)
Kang, Sung Gyun(강성균)Lee, Jung Hyun(이정현)
Publication Year
2011-09-06
Abstract
Thermococcus onnurineus NA1 is a typical sulfur-reducing hyperthermophilic archaeon. Genome sequence analysis has shown that T. onnurineus NA1 retains the metabolic pathways necessary not only for organotrophic, but also for carboxydotrophic, growth. T. onnurineus NA1 carboxydotrophic growth may result in hydrogen production, as this archaeon produces hydrogen during oxidizing formate. In this study, we profiled the proteome of T. onnurineus NA1 cultured under carboxydotrophic conditions using CO as an electron donor by the SDS-PAGE/LC-MS/MS method. A total of 1395 proteins were identified by two independent proteomic analyses, which corresponds to similar to 71% of the total predicted open reading frames. To our knowledge, this level of identification coverage exceeds those of other global proteome profiling studies in Archaea. Furthermore, the biological functions of the identified proteins were predicted and cognate enzymes were mapped to the appropriate metabolic pathways. More than 90% of the genes belonging to hydrogenase gene clusters such as Mbx, Sulf-I, Mbh, Hyg4-I, Hyg4-II, and Hyf4-III were expressed during CO culture. This means that hydrogenases induced under carboxydotrophic conditions surpass those induced under an organotrophic condition (yeast extract-peptone-sulfur). Our data suggest that hydrogen production is caused by the expression and functional assembly of T. onnurineus NA1 hydrogenase genes during culture in the presence of CO. (C) 2011 Elsevier B.V. All rights reserved.
ISSN
1874-3919
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3812
DOI
10.1016/j.jprot.2011.05.010
Bibliographic Citation
JOURNAL OF PROTEOMICS, v.74, no.10, pp.1926 - 1933, 2011
Publisher
ELSEVIER SCIENCE BV
Subject
SEA HYDROTHERMAL VENT; COMPLETE GENOME SEQUENCE; HYPERTHERMOPHILIC ARCHAEON; PEPTIDE IDENTIFICATION; PYROCOCCUS-FURIOSUS; SHOTGUN PROTEOMICS; KODAKARAENSIS KOD1; H-2 PRODUCTION; SP-NOV.; RUBRERYTHRIN
Keywords
SDS-PAGE/LC-MS/MS; Thermococcus onnurineus NA1; Carboxydotrophic growth
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER SCIENCE BV
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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