Development of DNA chip for jellyfish verification from South Korea SCIE SCOPUS KCI OTHER

Cited 4 time in WEB OF SCIENCE Cited 5 time in Scopus
Title
Development of DNA chip for jellyfish verification from South Korea
Author(s)
Lee, Gunsup; Park, So Yun; Hwang, Jinik; Lee, Youn-Ho; Hwang, Seung Yong; Lee, Sukchan; Lee, Taek-Kyun
KIOST Author(s)
Lee, Youn Ho(이윤호)Lee, Taek Kyun(이택견)
Publication Year
2011-12
Abstract
Global warming and environmental change have been responsible for an exponential increase in the number of jellyfish. The mass propagation of jellyfish has inflicted great damage to the economy worldwide. Therefore, in order to instantly determine the possible types of jellyfish, DNA barcode data analysis was conducted using molecular markers. DNA chip technology is an efficient method for species-level identification and, as such, will contribute powerfully to taxonomic and biodiversity research. In order to identify jellyfish species, the mitochondrial COI gene, which has been shown to be widely applicable in animal barcoding, was used; the selected species-specific probe was 23 bp and was printed onto silylated slides using a robotic microarrayer. Additionally, COI genes were amplified from the genomic DNA of jellyfish using the primers LCO1490 and HCO2198 targeted to COI. In this study, we amplified and analyzed species-specific COI sequences for six jellyfish species (Aequorea coerulescens, Aurelia aurita, Bolinopsis sp., Cyanea nozakii, Dactylometra quinquecirrha, Nemopilema nomurai) collected at Jangmok Bay in Geoje. Herein, we describe each of the jellyfish species detected via microarray analysis. As a result, the COI barcode sequence technique was found to be suitable for the identification of jellyfish from South Korea.
ISSN
1976-0280
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3789
DOI
10.1007/s13206-011-5412-9
Bibliographic Citation
BIOCHIP JOURNAL, v.5, no.4, pp.375 - 382, 2011
Publisher
KOREAN BIOCHIP SOCIETY-KBCS
Subject
SPECIES IDENTIFICATION; BARCODES; LIFE; INCREASES; TAXONOMY; GENETICS; BLOOMS; WATER; SEA
Keywords
Jellyfish; DNA chip; Mitochondrial COI gene; Barcode; Microarray analysis
Type
Article
Language
English
Document Type
Article
Publisher
KOREAN BIOCHIP SOCIETY-KBCS
Related Researcher
Research Interests

Marine Molecular Genetics,Marine Ecology and Evolution,해양분자유전,해양생태및진화

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse