Isolation and functional characterization of polyunsaturated fatty acid elongase (AsELOVL5) gene from black seabream (Acanthopagrus schlegelii) SCIE SCOPUS

Cited 19 time in WEB OF SCIENCE Cited 19 time in Scopus
Title
Isolation and functional characterization of polyunsaturated fatty acid elongase (AsELOVL5) gene from black seabream (Acanthopagrus schlegelii)
Author(s)
Kim, Sun Hee; Kim, Jong Bum; Jang, Yo Soon; Kim, So Yun; Roh, Kyung Hee; Kim, Hyun Uk; Lee, Kyeong-Ryeol; Park, Jong-Sug
Publication Year
2012-02
Abstract
To identify the genes encoding fatty acid elongases for the biosynthesis of polyunsaturated fatty acids (PUFAs), we isolated a cDNA via degenerate PCR and RACE-PCR from Acanthopagrus schlegelii with a high similarity to the ELOVL5-like elongases of mammals and fishes. This gene is termed AsELOVL5 and encodes a 294 amino acid protein. When AsELOVL5 was expressed in Saccharomyces cerevisiae, it conferred an ability to elongate gamma-linolenic acid (18:3 n-6) to di-homo-gamma-linolenic acid (20:3 n-6). In addition, the transformed cells converted arachidonic acid (20:4 n-6) and eicosapentaenpic acid (20:5 n-3) to docosatetraenoic acid (22:4 n-6) and docosapentaenoic acid (22:5 n-3), respectively. These results indicate that the AsELOVL5 gene encodes a long-chain fatty acid elongase capable of elongating C(18)Delta 6/C(20)Delta 5 but not C(22) PUFA substrates.
ISSN
0141-5492
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3635
DOI
10.1007/s10529-011-0746-x
Bibliographic Citation
BIOTECHNOLOGY LETTERS, v.34, no.2, pp.261 - 268, 2012
Publisher
SPRINGER
Subject
DESATURASE; IDENTIFICATION; EXPRESSION; CLONING; BIOSYNTHESIS; METABOLISM; DISEASE; MARINE; ROLES; CDNAS
Keywords
Acanthopagrus schlegelii; Polyunsaturated fatty acid elongase; Saccharomyces cerevisiae
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER
Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse