Isolation and functional characterization of polyunsaturated fatty acid elongase (AsELOVL5) gene from black seabream (Acanthopagrus schlegelii) SCIE SCOPUS
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- Title
- Isolation and functional characterization of polyunsaturated fatty acid elongase (AsELOVL5) gene from black seabream (Acanthopagrus schlegelii)
- KIOST Author(s)
- Jang, Yo Soon(장요순)
- Alternative Author(s)
- 장요순
- Publication Year
- 2012-02
- Abstract
- To identify the genes encoding fatty acid elongases for the biosynthesis of polyunsaturated fatty acids (PUFAs), we isolated a cDNA via degenerate PCR and RACE-PCR from Acanthopagrus schlegelii with a high similarity to the ELOVL5-like elongases of mammals and fishes. This gene is termed AsELOVL5 and encodes a 294 amino acid protein. When AsELOVL5 was expressed in Saccharomyces cerevisiae, it conferred an ability to elongate gamma-linolenic acid (18:3 n-6) to di-homo-gamma-linolenic acid (20:3 n-6). In addition, the transformed cells converted arachidonic acid (20:4 n-6) and eicosapentaenpic acid (20:5 n-3) to docosatetraenoic acid (22:4 n-6) and docosapentaenoic acid (22:5 n-3), respectively. These results indicate that the AsELOVL5 gene encodes a long-chain fatty acid elongase capable of elongating C(18)Delta 6/C(20)Delta 5 but not C(22) PUFA substrates.
- ISSN
- 0141-5492
- Bibliographic Citation
- BIOTECHNOLOGY LETTERS, v.34, no.2, pp.261 - 268, 2012
- Publisher
- SPRINGER
- Subject
- DESATURASE; IDENTIFICATION; EXPRESSION; CLONING; BIOSYNTHESIS; METABOLISM; DISEASE; MARINE; ROLES; CDNAS
- Keywords
- Acanthopagrus schlegelii; Polyunsaturated fatty acid elongase; Saccharomyces cerevisiae
- Type
- Article
- Language
- English
- Document Type
- Article
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- There are no files associated with this item.
