Isolation and functional characterization of polyunsaturated fatty acid elongase (AsELOVL5) gene from black seabream (Acanthopagrus schlegelii) SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Kim, Sun Hee | - |
dc.contributor.author | Kim, Jong Bum | - |
dc.contributor.author | Jang, Yo Soon | - |
dc.contributor.author | Kim, So Yun | - |
dc.contributor.author | Roh, Kyung Hee | - |
dc.contributor.author | Kim, Hyun Uk | - |
dc.contributor.author | Lee, Kyeong-Ryeol | - |
dc.contributor.author | Park, Jong-Sug | - |
dc.date.accessioned | 2020-04-20T06:55:40Z | - |
dc.date.available | 2020-04-20T06:55:40Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2012-02 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/3635 | - |
dc.description.abstract | To identify the genes encoding fatty acid elongases for the biosynthesis of polyunsaturated fatty acids (PUFAs), we isolated a cDNA via degenerate PCR and RACE-PCR from Acanthopagrus schlegelii with a high similarity to the ELOVL5-like elongases of mammals and fishes. This gene is termed AsELOVL5 and encodes a 294 amino acid protein. When AsELOVL5 was expressed in Saccharomyces cerevisiae, it conferred an ability to elongate gamma-linolenic acid (18:3 n-6) to di-homo-gamma-linolenic acid (20:3 n-6). In addition, the transformed cells converted arachidonic acid (20:4 n-6) and eicosapentaenpic acid (20:5 n-3) to docosatetraenoic acid (22:4 n-6) and docosapentaenoic acid (22:5 n-3), respectively. These results indicate that the AsELOVL5 gene encodes a long-chain fatty acid elongase capable of elongating C(18)Delta 6/C(20)Delta 5 but not C(22) PUFA substrates. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.subject | DESATURASE | - |
dc.subject | IDENTIFICATION | - |
dc.subject | EXPRESSION | - |
dc.subject | CLONING | - |
dc.subject | BIOSYNTHESIS | - |
dc.subject | METABOLISM | - |
dc.subject | DISEASE | - |
dc.subject | MARINE | - |
dc.subject | ROLES | - |
dc.subject | CDNAS | - |
dc.title | Isolation and functional characterization of polyunsaturated fatty acid elongase (AsELOVL5) gene from black seabream (Acanthopagrus schlegelii) | - |
dc.type | Article | - |
dc.citation.endPage | 268 | - |
dc.citation.startPage | 261 | - |
dc.citation.title | BIOTECHNOLOGY LETTERS | - |
dc.citation.volume | 34 | - |
dc.citation.number | 2 | - |
dc.contributor.alternativeName | 장요순 | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY LETTERS, v.34, no.2, pp.261 - 268 | - |
dc.identifier.doi | 10.1007/s10529-011-0746-x | - |
dc.identifier.scopusid | 2-s2.0-84856214560 | - |
dc.identifier.wosid | 000299510200011 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | DESATURASE | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | MARINE | - |
dc.subject.keywordPlus | ROLES | - |
dc.subject.keywordPlus | CDNAS | - |
dc.subject.keywordAuthor | Acanthopagrus schlegelii | - |
dc.subject.keywordAuthor | Polyunsaturated fatty acid elongase | - |
dc.subject.keywordAuthor | Saccharomyces cerevisiae | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |