Isolation and functional characterization of polyunsaturated fatty acid elongase (AsELOVL5) gene from black seabream (Acanthopagrus schlegelii) SCIE SCOPUS

DC Field Value Language
dc.contributor.author Kim, Sun Hee -
dc.contributor.author Kim, Jong Bum -
dc.contributor.author Jang, Yo Soon -
dc.contributor.author Kim, So Yun -
dc.contributor.author Roh, Kyung Hee -
dc.contributor.author Kim, Hyun Uk -
dc.contributor.author Lee, Kyeong-Ryeol -
dc.contributor.author Park, Jong-Sug -
dc.date.accessioned 2020-04-20T06:55:40Z -
dc.date.available 2020-04-20T06:55:40Z -
dc.date.created 2020-01-28 -
dc.date.issued 2012-02 -
dc.identifier.issn 0141-5492 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/3635 -
dc.description.abstract To identify the genes encoding fatty acid elongases for the biosynthesis of polyunsaturated fatty acids (PUFAs), we isolated a cDNA via degenerate PCR and RACE-PCR from Acanthopagrus schlegelii with a high similarity to the ELOVL5-like elongases of mammals and fishes. This gene is termed AsELOVL5 and encodes a 294 amino acid protein. When AsELOVL5 was expressed in Saccharomyces cerevisiae, it conferred an ability to elongate gamma-linolenic acid (18:3 n-6) to di-homo-gamma-linolenic acid (20:3 n-6). In addition, the transformed cells converted arachidonic acid (20:4 n-6) and eicosapentaenpic acid (20:5 n-3) to docosatetraenoic acid (22:4 n-6) and docosapentaenoic acid (22:5 n-3), respectively. These results indicate that the AsELOVL5 gene encodes a long-chain fatty acid elongase capable of elongating C(18)Delta 6/C(20)Delta 5 but not C(22) PUFA substrates. -
dc.description.uri 1 -
dc.language English -
dc.publisher SPRINGER -
dc.subject DESATURASE -
dc.subject IDENTIFICATION -
dc.subject EXPRESSION -
dc.subject CLONING -
dc.subject BIOSYNTHESIS -
dc.subject METABOLISM -
dc.subject DISEASE -
dc.subject MARINE -
dc.subject ROLES -
dc.subject CDNAS -
dc.title Isolation and functional characterization of polyunsaturated fatty acid elongase (AsELOVL5) gene from black seabream (Acanthopagrus schlegelii) -
dc.type Article -
dc.citation.endPage 268 -
dc.citation.startPage 261 -
dc.citation.title BIOTECHNOLOGY LETTERS -
dc.citation.volume 34 -
dc.citation.number 2 -
dc.contributor.alternativeName 장요순 -
dc.identifier.bibliographicCitation BIOTECHNOLOGY LETTERS, v.34, no.2, pp.261 - 268 -
dc.identifier.doi 10.1007/s10529-011-0746-x -
dc.identifier.scopusid 2-s2.0-84856214560 -
dc.identifier.wosid 000299510200011 -
dc.type.docType Article -
dc.description.journalClass 1 -
dc.subject.keywordPlus DESATURASE -
dc.subject.keywordPlus IDENTIFICATION -
dc.subject.keywordPlus EXPRESSION -
dc.subject.keywordPlus CLONING -
dc.subject.keywordPlus BIOSYNTHESIS -
dc.subject.keywordPlus METABOLISM -
dc.subject.keywordPlus DISEASE -
dc.subject.keywordPlus MARINE -
dc.subject.keywordPlus ROLES -
dc.subject.keywordPlus CDNAS -
dc.subject.keywordAuthor Acanthopagrus schlegelii -
dc.subject.keywordAuthor Polyunsaturated fatty acid elongase -
dc.subject.keywordAuthor Saccharomyces cerevisiae -
dc.relation.journalWebOfScienceCategory Biotechnology & Applied Microbiology -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.relation.journalResearchArea Biotechnology & Applied Microbiology -
Appears in Collections:
East Sea Research Institute > East Sea Environment Research Center > 1. Journal Articles
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