One-Step Sequence- and Ligation-Independent Cloning as a Rapid and Versatile Cloning Method for Functional Genomics Studies SCIE SCOPUS
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Jeong, Jae-Yeon | - |
| dc.contributor.author | Yim, Hyung-Soon | - |
| dc.contributor.author | Ryu, Ji-Young | - |
| dc.contributor.author | Lee, Hyun Sook | - |
| dc.contributor.author | Lee, Jung-Hyun | - |
| dc.contributor.author | Seen, Dong-Seung | - |
| dc.contributor.author | Kang, Sung Gyun | - |
| dc.date.accessioned | 2020-04-20T06:55:06Z | - |
| dc.date.available | 2020-04-20T06:55:06Z | - |
| dc.date.created | 2020-01-28 | - |
| dc.date.issued | 2012-08 | - |
| dc.identifier.issn | 0099-2240 | - |
| dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/3569 | - |
| dc.description.abstract | We developed one-step sequence- and ligation-independent cloning (SLIC) as a simple, cost-effective, time-saving, and versatile cloning method. Highly efficient and directional cloning can be achieved by direct bacterial transformation 2.5 min after mixing any linearized vector, an insert(s) prepared by PCR, and T4 DNA polymerase in a tube at room temperature. | - |
| dc.description.uri | 1 | - |
| dc.language | English | - |
| dc.publisher | AMER SOC MICROBIOLOGY | - |
| dc.subject | IN-VITRO | - |
| dc.subject | DNA | - |
| dc.subject | RECOMBINATION | - |
| dc.subject | VECTORS | - |
| dc.title | One-Step Sequence- and Ligation-Independent Cloning as a Rapid and Versatile Cloning Method for Functional Genomics Studies | - |
| dc.type | Article | - |
| dc.citation.endPage | 5443 | - |
| dc.citation.startPage | 5440 | - |
| dc.citation.title | APPLIED AND ENVIRONMENTAL MICROBIOLOGY | - |
| dc.citation.volume | 78 | - |
| dc.citation.number | 15 | - |
| dc.contributor.alternativeName | 정재연 | - |
| dc.contributor.alternativeName | 임형순 | - |
| dc.contributor.alternativeName | 류지영 | - |
| dc.contributor.alternativeName | 이현숙 | - |
| dc.contributor.alternativeName | 이정현 | - |
| dc.contributor.alternativeName | 강성균 | - |
| dc.identifier.bibliographicCitation | APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.78, no.15, pp.5440 - 5443 | - |
| dc.identifier.doi | 10.1128/AEM.00844-12 | - |
| dc.identifier.scopusid | 2-s2.0-84866171028 | - |
| dc.identifier.wosid | 000306532500051 | - |
| dc.type.docType | Article | - |
| dc.description.journalClass | 1 | - |
| dc.subject.keywordPlus | IN-VITRO | - |
| dc.subject.keywordPlus | DNA | - |
| dc.subject.keywordPlus | RECOMBINATION | - |
| dc.subject.keywordPlus | VECTORS | - |
| dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
| dc.relation.journalWebOfScienceCategory | Microbiology | - |
| dc.description.journalRegisteredClass | scie | - |
| dc.description.journalRegisteredClass | scopus | - |
| dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
| dc.relation.journalResearchArea | Microbiology | - |
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