One-Step Sequence- and Ligation-Independent Cloning as a Rapid and Versatile Cloning Method for Functional Genomics Studies SCIE SCOPUS

Cited 178 time in WEB OF SCIENCE Cited 183 time in Scopus
Title
One-Step Sequence- and Ligation-Independent Cloning as a Rapid and Versatile Cloning Method for Functional Genomics Studies
Author(s)
Jeong, Jae-Yeon; Yim, Hyung-Soon; Ryu, Ji-Young; Lee, Hyun Sook; Lee, Jung-Hyun; Seen, Dong-Seung; Kang, Sung Gyun
KIOST Author(s)
Yim, Hyung Soon(임형순)Lee, Hyun Sook(이현숙)Lee, Jung Hyun(이정현)Kang, Sung Gyun(강성균)
Publication Year
2012-08
Abstract
We developed one-step sequence- and ligation-independent cloning (SLIC) as a simple, cost-effective, time-saving, and versatile cloning method. Highly efficient and directional cloning can be achieved by direct bacterial transformation 2.5 min after mixing any linearized vector, an insert(s) prepared by PCR, and T4 DNA polymerase in a tube at room temperature.
ISSN
0099-2240
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3569
DOI
10.1128/AEM.00844-12
Bibliographic Citation
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.78, no.15, pp.5440 - 5443, 2012
Publisher
AMER SOC MICROBIOLOGY
Subject
IN-VITRO; DNA; RECOMBINATION; VECTORS
Type
Article
Language
English
Document Type
Article
Publisher
AMER SOC MICROBIOLOGY
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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