One-Step Sequence- and Ligation-Independent Cloning as a Rapid and Versatile Cloning Method for Functional Genomics Studies SCIE SCOPUS
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- Title
- One-Step Sequence- and Ligation-Independent Cloning as a Rapid and Versatile Cloning Method for Functional Genomics Studies
- KIOST Author(s)
- Yim, Hyung Soon(임형순); Lee, Hyun Sook(이현숙); Lee, Jung Hyun(이정현); Kang, Sung Gyun(강성균)
- Alternative Author(s)
- 정재연; 임형순; 류지영; 이현숙; 이정현; 강성균
- Publication Year
- 2012-08
- Abstract
- We developed one-step sequence- and ligation-independent cloning (SLIC) as a simple, cost-effective, time-saving, and versatile cloning method. Highly efficient and directional cloning can be achieved by direct bacterial transformation 2.5 min after mixing any linearized vector, an insert(s) prepared by PCR, and T4 DNA polymerase in a tube at room temperature.
- ISSN
- 0099-2240
- Bibliographic Citation
- APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.78, no.15, pp.5440 - 5443, 2012
- Publisher
- AMER SOC MICROBIOLOGY
- Subject
- IN-VITRO; DNA; RECOMBINATION; VECTORS
- Type
- Article
- Language
- English
- Document Type
- Article
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- There are no files associated with this item.
