Crystallization and preliminary X-ray crystallographic analysis of the putative NADP(H)-dependent oxidoreductase YncB from Vibrio vulnificus SCIE SCOPUS
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Min-Kyu | - |
dc.contributor.author | An, Young Jun | - |
dc.contributor.author | Jeong, Chang-Sook | - |
dc.contributor.author | Cha, Sun-Shin | - |
dc.date.accessioned | 2020-04-20T06:40:43Z | - |
dc.date.available | 2020-04-20T06:40:43Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2012-09 | - |
dc.identifier.issn | 2053-230X | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/3468 | - |
dc.description.abstract | The yncB gene product from Vibrio vulnificus, which belongs to the medium-chain dehydrogenase/reductase (MDR) superfamily, was crystallized using the microbatch crystallization method at 295 K. Diffraction data sets were collected using synchrotron radiation. Crystals of selenomethionine-substituted YncB protein belonged to space group P212121, with unit-cell parameters a = 90.52, b=91.56, c = 104.79 angstrom. Assuming the presence of two molecules in the asymmetric unit, the solvent content was estimated to be about 57%. Crystals of the YncBNADP(H) complex belonged to space group P41212 or P43212, with unit-cell parameters a = b = 90.14, c = 105.61 angstrom. Assuming the presence of one molecule in the asymmetric unit, the solvent content was estimated to be about 56.42%. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | INT UNION CRYSTALLOGRAPHY | - |
dc.title | Crystallization and preliminary X-ray crystallographic analysis of the putative NADP(H)-dependent oxidoreductase YncB from Vibrio vulnificus | - |
dc.type | Article | - |
dc.citation.endPage | 1101 | - |
dc.citation.startPage | 1098 | - |
dc.citation.title | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | - |
dc.citation.volume | 68 | - |
dc.contributor.alternativeName | 김민규 | - |
dc.contributor.alternativeName | 안영준 | - |
dc.contributor.alternativeName | 정창숙 | - |
dc.contributor.alternativeName | 차선신 | - |
dc.identifier.bibliographicCitation | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, v.68, pp.1098 - 1101 | - |
dc.identifier.doi | 10.1107/S1744309112030527 | - |
dc.identifier.scopusid | 2-s2.0-84865709383 | - |
dc.identifier.wosid | 000308407200023 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.subject.keywordPlus | MEDIUM-CHAIN DEHYDROGENASES/REDUCTASES | - |
dc.subject.keywordPlus | QUINONE OXIDOREDUCTASE | - |
dc.subject.keywordPlus | ALCOHOL DEHYDROGENASES | - |
dc.subject.keywordPlus | MDR SUPERFAMILY | - |
dc.subject.keywordPlus | CURCUMIN | - |
dc.subject.keywordPlus | FAMILY | - |
dc.subject.keywordPlus | TETRAHYDROCURCUMIN | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | RATS | - |
dc.subject.keywordAuthor | MDR superfamily | - |
dc.subject.keywordAuthor | Vibrio vulnificus | - |
dc.subject.keywordAuthor | YncB | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Crystallography | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Crystallography | - |