Crystallization and preliminary X-ray crystallographic analysis of the putative NADP(H)-dependent oxidoreductase YncB from Vibrio vulnificus
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Title
- Crystallization and preliminary X-ray crystallographic analysis of the putative NADP(H)-dependent oxidoreductase YncB from Vibrio vulnificus
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Author(s)
- Kim, Min-Kyu; An, Young Jun; Jeong, Chang-Sook; Cha, Sun-Shin
- KIOST Author(s)
- An, Young Jun(안영준)
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Alternative Author(s)
- 김민규; 안영준; 정창숙; 차선신
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Publication Year
- 2012-09
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Abstract
- The yncB gene product from Vibrio vulnificus, which belongs to the medium-chain dehydrogenase/reductase (MDR) superfamily, was crystallized using the microbatch crystallization method at 295 K. Diffraction data sets were collected using synchrotron radiation. Crystals of selenomethionine-substituted YncB protein belonged to space group P212121, with unit-cell parameters a = 90.52, b=91.56, c = 104.79 angstrom. Assuming the presence of two molecules in the asymmetric unit, the solvent content was estimated to be about 57%. Crystals of the YncBNADP(H) complex belonged to space group P41212 or P43212, with unit-cell parameters a = b = 90.14, c = 105.61 angstrom. Assuming the presence of one molecule in the asymmetric unit, the solvent content was estimated to be about 56.42%.
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ISSN
- 2053-230X
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/3468
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DOI
- 10.1107/S1744309112030527
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Bibliographic Citation
- ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, v.68, pp.1098 - 1101, 2012
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Publisher
- INT UNION CRYSTALLOGRAPHY
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Keywords
- MDR superfamily; Vibrio vulnificus; YncB
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Type
- Article
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Language
- English
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Document Type
- Article
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