Experimental phasing using zinc anomalous scattering SCIE SCOPUS

Cited 22 time in WEB OF SCIENCE Cited 21 time in Scopus
Title
Experimental phasing using zinc anomalous scattering
Author(s)
Cha, Sun-Shin; An, Young Jun; Jeong, Chang-Sook; Kim, Min-Kyu; Lee, Sung-Gyu; Lee, Kwang-Hoon; Oh, Byung-Ha
KIOST Author(s)
An, Young Jun(안영준)
Alternative Author(s)
차선신; 안영준; 정창숙; 김민규
Publication Year
2012-09
Abstract
Zinc is a suitable metal for anomalous dispersion phasing methods in protein crystallography. Structure determination using zinc anomalous scattering has been almost exclusively limited to proteins with intrinsically bound zinc(s). Here, it is reported that multiple zinc ions can easily be charged onto the surface of proteins with no intrinsic zinc-binding site by using zinc-containing solutions. Zn derivatization of protein surfaces appears to be a largely unnoticed but promising method of protein structure determination.
ISSN
0907-4449
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3466
DOI
10.1107/S0907444912024420
Bibliographic Citation
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, v.68, pp.1253 - 1258, 2012
Publisher
INT UNION CRYSTALLOGRAPHY
Keywords
phasing; zinc anomalous scattering; Zn derivatization
Type
Article
Language
English
Document Type
Article
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