Experimental phasing using zinc anomalous scattering
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Title
- Experimental phasing using zinc anomalous scattering
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Author(s)
- Cha, Sun-Shin; An, Young Jun; Jeong, Chang-Sook; Kim, Min-Kyu; Lee, Sung-Gyu; Lee, Kwang-Hoon; Oh, Byung-Ha
- KIOST Author(s)
- An, Young Jun(안영준)
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Alternative Author(s)
- 차선신; 안영준; 정창숙; 김민규
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Publication Year
- 2012-09
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Abstract
- Zinc is a suitable metal for anomalous dispersion phasing methods in protein crystallography. Structure determination using zinc anomalous scattering has been almost exclusively limited to proteins with intrinsically bound zinc(s). Here, it is reported that multiple zinc ions can easily be charged onto the surface of proteins with no intrinsic zinc-binding site by using zinc-containing solutions. Zn derivatization of protein surfaces appears to be a largely unnoticed but promising method of protein structure determination.
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ISSN
- 0907-4449
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/3466
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DOI
- 10.1107/S0907444912024420
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Bibliographic Citation
- ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, v.68, pp.1253 - 1258, 2012
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Publisher
- INT UNION CRYSTALLOGRAPHY
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Keywords
- phasing; zinc anomalous scattering; Zn derivatization
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Type
- Article
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Language
- English
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Document Type
- Article
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