Development of DNA chip for verification of 25 microalgae collected from southern coastal region in Korea SCIE SCOPUS KCI OTHER

Cited 3 time in WEB OF SCIENCE Cited 3 time in Scopus
Title
Development of DNA chip for verification of 25 microalgae collected from southern coastal region in Korea
Author(s)
Lee, Gunsup; Park, So Yun; Yum, Seungshic; Woo, Seonock; Lee, Youn-Ho; Hwang, Seung Yong; Park, Heung-Sik; Moh, Sang Hyun; Lee, Sukchan; Lee, Taek-Kyun
KIOST Author(s)
Yum, Seung Shic(염승식)Lee, Youn Ho(이윤호)Park, Heung Sik(박흥식)Lee, Taek Kyun(이택견)
Publication Year
2012-12
Abstract
Countless species occur in the marine microalgal domain. Some are used as health functional foods or medical products but many species are harmful such as those that cause the red tide. Therefore, it is necessary to conduct prompt and accurate identification of microalgal species. As it is quite difficult to accurately distinguish all species in terms of morphology, we performed DNA barcoding analysis using molecular markers for more accurate and rapid screening. DNA barcoding analysis, i.e., DNA chip technology, is a powerful method for studies on microalgal taxonomy and biodiversity. We used the mitochondrial cytochrome c oxidase subunit I (mtCOI) as a barcoding gene to identify microalgal species. In this study, the diversity and phylogenetic differences among different microalgae were analyzed. Additionally, a microalgal species-specific probe was screened by 21-23 bp and the result was printed on silylated slide for use in a robotic microarrayer. As a result, we performed a DNA chip assay for each of 25 microalgal species and determined that the COI barcode gene was suitable as a marker gene, as it could identify various microalgae from the Korean South Sea by species.
ISSN
1976-0280
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3417
DOI
10.1007/s13206-012-6404-0
Bibliographic Citation
BIOCHIP JOURNAL, v.6, no.4, pp.325 - 334, 2012
Publisher
KOREAN BIOCHIP SOCIETY-KBCS
Subject
SPECIES IDENTIFICATION; BARCODES; MARKER; WATER; LIFE; RED
Keywords
Microalgae; DNA barcoding; Mitochondrial cytochrome c oxidase subunit I; DNA chip assay; Marker gene
Type
Article
Language
English
Document Type
Article
Publisher
KOREAN BIOCHIP SOCIETY-KBCS
Related Researcher
Research Interests

Marine Molecular Genetics,Marine Ecology and Evolution,해양분자유전,해양생태및진화

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