Development of DNA chip for verification of 25 microalgae collected from southern coastal region in Korea SCIE SCOPUS KCI OTHER

DC Field Value Language Lee, Gunsup - Park, So Yun - Yum, Seungshic - Woo, Seonock - Lee, Youn-Ho - Hwang, Seung Yong - Park, Heung-Sik - Moh, Sang Hyun - Lee, Sukchan - Lee, Taek-Kyun - 2020-04-20T06:40:17Z - 2020-04-20T06:40:17Z - 2020-01-28 - 2012-12 -
dc.identifier.issn 1976-0280 -
dc.identifier.uri -
dc.description.abstract Countless species occur in the marine microalgal domain. Some are used as health functional foods or medical products but many species are harmful such as those that cause the red tide. Therefore, it is necessary to conduct prompt and accurate identification of microalgal species. As it is quite difficult to accurately distinguish all species in terms of morphology, we performed DNA barcoding analysis using molecular markers for more accurate and rapid screening. DNA barcoding analysis, i.e., DNA chip technology, is a powerful method for studies on microalgal taxonomy and biodiversity. We used the mitochondrial cytochrome c oxidase subunit I (mtCOI) as a barcoding gene to identify microalgal species. In this study, the diversity and phylogenetic differences among different microalgae were analyzed. Additionally, a microalgal species-specific probe was screened by 21-23 bp and the result was printed on silylated slide for use in a robotic microarrayer. As a result, we performed a DNA chip assay for each of 25 microalgal species and determined that the COI barcode gene was suitable as a marker gene, as it could identify various microalgae from the Korean South Sea by species. -
dc.description.uri 1 -
dc.language English -
dc.subject BARCODES -
dc.subject MARKER -
dc.subject WATER -
dc.subject LIFE -
dc.subject RED -
dc.title Development of DNA chip for verification of 25 microalgae collected from southern coastal region in Korea -
dc.type Article -
dc.citation.endPage 334 -
dc.citation.startPage 325 -
dc.citation.title BIOCHIP JOURNAL -
dc.citation.volume 6 -
dc.citation.number 4 -
dc.identifier.bibliographicCitation BIOCHIP JOURNAL, v.6, no.4, pp.325 - 334 -
dc.identifier.doi 10.1007/s13206-012-6404-0 -
dc.identifier.scopusid 2-s2.0-84871170079 -
dc.identifier.wosid 000312401100004 -
dc.type.docType Article -
dc.identifier.kciid ART001719591 -
dc.description.journalClass 1 -
dc.subject.keywordPlus SPECIES IDENTIFICATION -
dc.subject.keywordPlus BARCODES -
dc.subject.keywordPlus MARKER -
dc.subject.keywordPlus WATER -
dc.subject.keywordPlus LIFE -
dc.subject.keywordPlus RED -
dc.subject.keywordAuthor Microalgae -
dc.subject.keywordAuthor DNA barcoding -
dc.subject.keywordAuthor Mitochondrial cytochrome c oxidase subunit I -
dc.subject.keywordAuthor DNA chip assay -
dc.subject.keywordAuthor Marker gene -
dc.relation.journalWebOfScienceCategory Biochemical Research Methods -
dc.relation.journalWebOfScienceCategory Chemistry, Analytical -
dc.relation.journalWebOfScienceCategory Nanoscience & Nanotechnology -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.description.journalRegisteredClass kci -
dc.description.journalRegisteredClass other -
dc.relation.journalResearchArea Biochemistry & Molecular Biology -
dc.relation.journalResearchArea Chemistry -
dc.relation.journalResearchArea Science & Technology - Other Topics -
Appears in Collections:
Marine Environmental & Climate Research Division > Marine Ecosystem Research Center > 1. Journal Articles
Marine Resources Research Division > Marine Biotechnology Research Center > 1. Journal Articles
Marine Resources Research Division > Marine Bio-Resources Research Unit > 1. Journal Articles
South Sea Research Institute > Risk Assessment Research Center > 1. Journal Articles
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