저온으로 유도된 넙치의 인공 동면 중 단백질 변화 양상

Abstract

To investigate the key proteins roled in the hibernation of flat fish and rockfish, this study evaluated the effect of induced hybernation caused by starvation at low temperature on the expressed protein pattern in muscle and liver in Paralichthys olivaceus and Sebastes pachycephalus. A series of 2-D electrophoresis works as well as HPLC analysis were performed with protein samples isolated from the muscle and liver of specimens maintained at 5, 15, and 25 oC sea water bath, with and without food supply. For 20 days of hybernation induction periods, a series of proteins of marked difference in expression level were recognized.