해양미생물 Vibrio sp. 98CJ11027에서 특이한 Chitinase 유전자의 분리

Title
해양미생물 Vibrio sp. 98CJ11027에서 특이한 Chitinase 유전자의 분리
Alternative Title
Molecular Cloning and Expression of a Novel Chitinase Gene from Vibrio sp. 98CJ11027
Author(s)
이재형; 이유경; 이홍금
Alternative Author(s)
이재형; 이유경; 이홍금
Publication Year
2004-05-13
Abstract
The chiK gene encoding chitinase ChiK was isolated from a marine bacterium, Vibrio sp. 98CJ11027. We amplified a partial sequence of the chiK using chitinase-specific primer sets. Using inverse PCR cloning method with two specific sets of PCR primers rather than single set, we identified the 4 kb DNA fragment containing chiK gene. From the fragment, we amplified the full chiK gene and cloned into pUC18 vector designated as pChiK. The recombinant E. coli clones harboring pChiK produced a clear zone around the colony in the colloidal chitin plates. Open reading frame of the chiK is 2553 bp long encoding a ChiK precursor protein of 851 amino acids, which consist of N-terminal signal peptide of 21 amino acids and a mature ChiK protein with 830 amino acids. The deduced amino acid sequences of chiK shows 89% identity with that of Vibrio carchariae chitinase. The conserved module of Vibrio sp. 98CJ11027 ChiK indicates that the ChiK belongs to the family 18 of glycoside hydrolases.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/31937
Bibliographic Citation
International Meeting of the Microbiological Society of Korea, pp.218, 2004
Publisher
한국미생물학회
Type
Conference
Language
English
Publisher
한국미생물학회
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