해양미생물 Vibrio sp. 98CJ11027에서 특이한 Chitinase 유전자의 분리
DC Field | Value | Language |
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dc.contributor.author | 이재형 | - |
dc.contributor.author | 이유경 | - |
dc.contributor.author | 이홍금 | - |
dc.date.accessioned | 2020-07-17T11:30:23Z | - |
dc.date.available | 2020-07-17T11:30:23Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2004-05-13 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/31937 | - |
dc.description.abstract | The chiK gene encoding chitinase ChiK was isolated from a marine bacterium, Vibrio sp. 98CJ11027. We amplified a partial sequence of the chiK using chitinase-specific primer sets. Using inverse PCR cloning method with two specific sets of PCR primers rather than single set, we identified the 4 kb DNA fragment containing chiK gene. From the fragment, we amplified the full chiK gene and cloned into pUC18 vector designated as pChiK. The recombinant E. coli clones harboring pChiK produced a clear zone around the colony in the colloidal chitin plates. Open reading frame of the chiK is 2553 bp long encoding a ChiK precursor protein of 851 amino acids, which consist of N-terminal signal peptide of 21 amino acids and a mature ChiK protein with 830 amino acids. The deduced amino acid sequences of chiK shows 89% identity with that of Vibrio carchariae chitinase. The conserved module of Vibrio sp. 98CJ11027 ChiK indicates that the ChiK belongs to the family 18 of glycoside hydrolases. | - |
dc.description.uri | 2 | - |
dc.language | English | - |
dc.publisher | 한국미생물학회 | - |
dc.relation.isPartOf | International Meeting of the Microbiological Society of Korea | - |
dc.title | 해양미생물 Vibrio sp. 98CJ11027에서 특이한 Chitinase 유전자의 분리 | - |
dc.title.alternative | Molecular Cloning and Expression of a Novel Chitinase Gene from Vibrio sp. 98CJ11027 | - |
dc.type | Conference | - |
dc.citation.conferencePlace | KO | - |
dc.citation.endPage | 218 | - |
dc.citation.startPage | 218 | - |
dc.citation.title | International Meeting of the Microbiological Society of Korea | - |
dc.contributor.alternativeName | 이재형 | - |
dc.contributor.alternativeName | 이유경 | - |
dc.contributor.alternativeName | 이홍금 | - |
dc.identifier.bibliographicCitation | International Meeting of the Microbiological Society of Korea, pp.218 | - |
dc.description.journalClass | 2 | - |