Molecular Cloning and Enzymatic Characterization of Cyclomalto-dextrinase from Hyperthermophilic Archaeon Thermococcus sp CL1 SCIE SCOPUS KCI

Cited 11 time in WEB OF SCIENCE Cited 11 time in Scopus
Title
Molecular Cloning and Enzymatic Characterization of Cyclomalto-dextrinase from Hyperthermophilic Archaeon Thermococcus sp CL1
Author(s)
Lee, Jae-Eun; Kim, In-Hwan; Jung, Jong-Hyun; Seo, Dong-Ho; Kang, Sung-Gyun; Holden, James F.; Cha, Jaeho; Park, Cheon-Seok
KIOST Author(s)
Kang, Sung Gyun(강성균)
Publication Year
2013-08
Abstract
Genome organization near cyclomaltodextrinases (CDases) was analyzed and compared for four different hyperthermophilic archaea: Thermococcus, Pyrococcus, Staphylothermus, and Thermofilum. A gene (CL1_0884) encoding a putative CDase from Thermococcus sp. CL1 (tccd) was cloned and expressed in Escherichia coli. TcCD was confirmed to be highly thermostable, with optimal activity at 85 degrees C. The melting temperature of TcCD was determined to be 93 degrees C by both differential scanning calorimetry and differential scanning fluorimetry. A size-exclusion chromatography experiment showed that TcCD exists as a monomer. TcCD preferentially hydrolyzed alpha-cyclodextrin (alpha-CD), and at the initial stage catalyzed a ring-opening reaction by cleaving one alpha-1,4-glycosidic linkage of the CD ring to produce the corresponding single maltooligosaccharide. Furthermore, TcCD could hydrolyze branched CDs (G1-alpha-CD, G1-beta-CD, and G2-beta-CD) to yield significant amounts (45%, 40%, and 46%) of isomaltooligosaccharides (panose and 6(2)-alpha-maltosylmaltose) in addition to glucose and maltose. This enzyme is one of the most thermostable maltogenic amylases reported, and might be of potential value in the production of isomaltooligosaccharides in the food industry.
ISSN
1017-7825
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3137
DOI
10.4014/jmb.1302.02073
Bibliographic Citation
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.23, no.8, pp.1060 - 1069, 2013
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Subject
COMPLETE GENOME SEQUENCE; MALTOGENIC AMYLASE; AMYLOLYTIC ENZYME; ALPHA-AMYLASE; METABOLISM
Keywords
Cyclomaltodextrinase; isomaltooligosaccharides; panose; Thermococcus
Type
Article
Language
English
Document Type
Article
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Related Researcher
Research Interests

marine biotechnology,microbiology,해양생명공학,미생물학

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