메타게놈 연구를 통한 해양미생물 다양성의 추정과 이용

Title
메타게놈 연구를 통한 해양미생물 다양성의 추정과 이용
Alternative Title
Assessment and utilization of marine microbial diversity by using metagenomic study
Author(s)
이정현; 전정호; 이현숙; 배승섭; 임재규; 강성균; 김상진
KIOST Author(s)
Lee, Jung Hyun(이정현)Lee, Hyun Sook(이현숙)Kang, Sung Gyun(강성균)
Publication Year
2005-10-13
Abstract
Recent studies on marine environments have led to significant discoveries of unusual microbial diversity, genetic potential and metabolic activity in a variety of marine habitats; cold waters, sinking particles, sediments, and animal guts or other biological surfaces (at temperatures from -1.7℃ in polar oceans to ~4℃ at temperate and tropical latitudes); hydrothermal vent environments that encompass a wide range of temperatures (2-350℃) and nutritional conditions (organic compounds, reduced chemicals, heavy metals); and seafloor habitats in deeply buried sediments (>500m below the seafloor). Among the various microbial habitats, the marine sediment of coastal area and deep sea are considered to be eutrophic habitats rich in nutrients which may contain higher level of microbial diversity. The access of microbial diversity by culture techniques has been limited due to low cultivability less than 1%. An alternative procedure for the obtaining of the collective microbial genome in a given habitat (termed as metagenome) has been extensively studied; i.e. extracting total DNA and cloning it into proper vector systems.We have collected samples from various sediments of deep-sea clam beds community (depth, 1,400 m) in low-temperature venting area located in western Pacific area, intertidal zone of Ganghwa-island, and coasts of Korean Arctic Station, Dasan in Svalbard, Norway. The environmental DNAs of high quality and long size (>30 kb) were cloned into a fosmid vector system. The library contained 220,000 clones with average size of 31 kb, which equivalent to 6,820 Mb in total. The individual clone in the metagenomic library from a special microbial community of clam beds area has been analyzed by end-sequencing and useful enzymatic activity was screened. The end-sequences (~500 bp in length) of 223 clones were obtained and sequence analysis was accomplished. The sequences of 101 clones (45%) have showed similarity to proteins from γ-Proteobacteria (32%), α-Proteoba
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/31296
Bibliographic Citation
한국미생물연합회 프로시딩, pp.107, 2005
Publisher
한국미생물연합회
Type
Conference
Language
English
Publisher
한국미생물연합회
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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