Expression at 279 K, purification, crystallization and preliminary X-ray crystallographic analysis of a novel cold-active beta-1,4-D-mannanase from the Antarctic springtail Cryptopygus antarcticus
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Title
- Expression at 279 K, purification, crystallization and preliminary X-ray crystallographic analysis of a novel cold-active beta-1,4-D-mannanase from the Antarctic springtail Cryptopygus antarcticus
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Author(s)
- Kim, Min-Kyu; An, Young Jun; Jeong, Chang-Sook; Song, Jung Min; Kang, Mee Hye; Lee, Youn-Ho; Cha, Sun-Shin
- KIOST Author(s)
- An, Young Jun(안영준); Lee, Youn Ho(이윤호)
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Alternative Author(s)
- 김민규; 안영준; 정창숙; 송정민; 강미혜; 이윤호; 차선신
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Publication Year
- 2013-09
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Abstract
- The CaMan gene product from Cryptopygus antarcticus, which belongs to the glycoside hydrolase family 5 type beta-1,4-D-mannanases, has been crystallized using a precipitant solution consisting of 0.1 M Tris-HCl pH 8.5, 25%(w/v) polyethylene glycol 3350 by the microbatch crystallization method at 295 K. The CaMan protein crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 73.40, b = 83.81, c = 163.55 angstrom. Assuming the presence of two molecules in the asymmetric unit, the solvent content was estimated to be about 61.29%. CaMan-mannopentaose (M5) complex crystals that were isomorphous to the CaMan crystals were obtained using the same mother liquor containing 1 mM M5.
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ISSN
- 2053-230X
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/3113
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DOI
- 10.1107/S1744309113020538
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Bibliographic Citation
- ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, v.69, pp.1007 - 1010, 2013
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Publisher
- INT UNION CRYSTALLOGRAPHY
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Keywords
- β-1,4-d-mannanase; Cryptopygus antarcticus; glycoside hydrolase family 5
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Type
- Article
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Language
- English
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Document Type
- Article
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