Experimental Phasing Using Zinc and Sulfur Anomalous Signals Measured at the Zinc Absorption Peak
SCIE
SCOPUS
KCI
Cited 1 time in
WEB OF SCIENCE
Cited 0 time in
Scopus
-
Title
- Experimental Phasing Using Zinc and Sulfur Anomalous Signals Measured at the Zinc Absorption Peak
-
Author(s)
- Lee, Sangmin; Kim, Min-Kyu; Ji, Chang-Jun; Lee, Jin-Won; Cha, Sun-Shin
-
Alternative Author(s)
- 이상민; 김민규; 차선신
-
Publication Year
- 2013-10
-
Abstract
- Iron is an essential transition metal required for bacterial growth and survival. Excess free iron can lead to the generation of reactive oxygen species that can cause severe damage to cellular functions. Cells have developed iron-sensing regulators to maintain iron homeostasis at the transcription level. The ferric uptake regulator (Fur) is an iron-responsive regulator that controls the expression of genes involved in iron homeostasis, bacterial virulence, stress resistance, and redox metabolism. Here, we report the expression, purification, crystallization, and phasing of the apo-form of Bacillus subtilis Fur (BsFur) in the absence of regulatory metal ions. Crystals were obtained by microbatch crystallization method at 295 K and diffraction data at a resolution of 2.6 angstrom was collected at the zinc peak wavelength (lambda=1.2823 angstrom). Experimental phasing identified the positions of one zinc atom and four sulfur atoms of cysteine residues coordinating the zinc atom, indicating that the data contained a meaningful anomalous scattering originating from the ordered zinc-coordinating sulfur atoms, in spite of the small anomalous signals of sulfur atoms at the examined wavelength.
-
ISSN
- 1225-8873
-
URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/3092
-
DOI
- 10.1007/s12275-013-3412-2
-
Bibliographic Citation
- JOURNAL OF MICROBIOLOGY, v.51, no.5, pp.639 - 643, 2013
-
Publisher
- MICROBIOLOGICAL SOCIETY KOREA
-
Subject
- AUTOMATED STRUCTURE SOLUTION; CRYSTAL-STRUCTURE; IRON HOMEOSTASIS; FUR; EXPRESSION; PROTEIN; GENE; TRANSCRIPTION; BINDING; SYSTEM
-
Keywords
- Ferric uptake regulator; transcription regulator; crystallization; experimental phasing
-
Type
- Article
-
Language
- English
-
Document Type
- Article
- Files in This Item:
-
There are no files associated with this item.
Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.