The crystal structure of a novel phosphopantothenate synthetase from the hyperthermophilic archaea, Thermococcus onnurineus NA1
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Title
- The crystal structure of a novel phosphopantothenate synthetase from the hyperthermophilic archaea, Thermococcus onnurineus NA1
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Author(s)
- Kim, Min-Kyu; An, Young Jun; Cha, Sun-Shin
- KIOST Author(s)
- An, Young Jun(안영준)
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Alternative Author(s)
- 김민규; 안영준; 차선신
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Publication Year
- 2013-10-04
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Abstract
- Pantothenate is the essential precursor of coenzyme A (CoA), a fundamental cofactor in all aspects of metabolism. In bacteria and eukaryotes, pantothenate synthetase (PS) catalyzes the last step in the pantothenate biosynthetic pathway, and pantothenate kinase (PanK) phosphorylates pantothenate for its entry into the CoA biosynthetic pathway. However, genes encoding PS and PanK have not been identified in archaeal genomes. Recently, a comparative genomic analysis and the identification and characterization of two novel archaea-specific enzymes show that archaeal pantoate kinase (PoK) and phosphgpantothenate synthetase (PPS) represent counterparts to the PS/PanK pathway in bacteria and eukaryotes. The TON1374 protein from Thermococcus onnurineus NM is a PPS, that shares 54% sequence identity with the first reported archaeal PPS candidate, MM2281, from Methanosarcina mazei and 91% sequence identity with TK1686, the PPS from Thermococcus kodakarensis. Here, we report the apo and ATP-complex structures of TON1374 and discuss the substrate-binding mode and reaction mechanism. (C) 2013 Elsevier Inc. All rights reserved.
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ISSN
- 0006-291X
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/3080
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DOI
- 10.1016/j.bbrc.2013.09.008
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Bibliographic Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.439, no.4, pp.533 - 538, 2013
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Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
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Subject
- COENZYME-A BIOSYNTHESIS; BIOCHEMICAL-CHARACTERIZATION; PANTOTHENATE SYNTHETASE; ENZYME; FAMILIES; SYSTEM; SUITE; ACID
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Keywords
- Coenzyme A biosynthesis; Archaea; Phosphopantothenate synthetase; Crystal structure of TON1374; Crystal structure of TON1374/ATP complex
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Type
- Article
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Language
- English
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Document Type
- Article
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