A nucleic acid hydrolyzing recombinant antibody confers resistance to curtovirus infection in tobacco SCIE SCOPUS

Cited 13 time in WEB OF SCIENCE Cited 13 time in Scopus
Title
A nucleic acid hydrolyzing recombinant antibody confers resistance to curtovirus infection in tobacco
Author(s)
Lee, Gunsup; Shim, Hye-Kyung; Kwon, Myung-Hee; Son, Su-Hwan; Kim, Ki-Yoon; Park, Eul-Yong; Lee, Taek-Kyun; Lee, Woo-Ram; Auh, Chung-Kyoon; Kim, Donggiun; Kim, Yong-Sung; Lee, Sukchan
KIOST Author(s)
Lee, Taek Kyun(이택견)
Publication Year
2013-11
Abstract
A catalytic single chain variable antibody (scFv), 3D8 scFv, which has DNase activities, was functionally expressed in Nicotiana tabacum. The subcellular localization of the GFP-fused 3D8 indicated that the 3D8 protein was expressed in the cytosol of the N. tabacum protoplasts. Progenies of the transgenic tobacco plants exhibited complete resistance against two single stranded (ss) DNA geminiviruses, including the Beet curly top virus and the Beet severe curly top virus, without viral accumulation or disease symptoms. We presented a novel strategy for targeting the viral DNA itself in a sequence non-specific manner, rather than the viral proteins or RNAs, in order to generate virus-resistant transgenic plants. No noticeable adverse effects on the growth and reproduction of the transgenic plants were observed. Our results demonstrated that targeting viral DNA is an effective strategy for protecting plants from ssDNA viruses.
ISSN
0167-6857
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3065
DOI
10.1007/s11240-013-0357-4
Bibliographic Citation
PLANT CELL TISSUE AND ORGAN CULTURE, v.115, no.2, pp.179 - 187, 2013
Publisher
SPRINGER
Subject
PATHOGEN-DERIVED RESISTANCE; DEPENDENT RNA-POLYMERASE; LEAF CURL GEMINIVIRUS; SINGLE-CHAIN FV; TRANSGENIC PLANTS; NICOTIANA-BENTHAMIANA; VIRUS-RESISTANCE; PROTEIN; GENE; DNA
Keywords
Antibody; 3D8 scFv; DNase; Geminivirus; Nicotiana tabacum
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER
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