A nucleic acid hydrolyzing recombinant antibody confers resistance to curtovirus infection in tobacco SCIE SCOPUS

DC Field Value Language
dc.contributor.author Lee, Gunsup -
dc.contributor.author Shim, Hye-Kyung -
dc.contributor.author Kwon, Myung-Hee -
dc.contributor.author Son, Su-Hwan -
dc.contributor.author Kim, Ki-Yoon -
dc.contributor.author Park, Eul-Yong -
dc.contributor.author Lee, Taek-Kyun -
dc.contributor.author Lee, Woo-Ram -
dc.contributor.author Auh, Chung-Kyoon -
dc.contributor.author Kim, Donggiun -
dc.contributor.author Kim, Yong-Sung -
dc.contributor.author Lee, Sukchan -
dc.date.accessioned 2020-04-20T05:25:23Z -
dc.date.available 2020-04-20T05:25:23Z -
dc.date.created 2020-01-28 -
dc.date.issued 2013-11 -
dc.identifier.issn 0167-6857 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/3065 -
dc.description.abstract A catalytic single chain variable antibody (scFv), 3D8 scFv, which has DNase activities, was functionally expressed in Nicotiana tabacum. The subcellular localization of the GFP-fused 3D8 indicated that the 3D8 protein was expressed in the cytosol of the N. tabacum protoplasts. Progenies of the transgenic tobacco plants exhibited complete resistance against two single stranded (ss) DNA geminiviruses, including the Beet curly top virus and the Beet severe curly top virus, without viral accumulation or disease symptoms. We presented a novel strategy for targeting the viral DNA itself in a sequence non-specific manner, rather than the viral proteins or RNAs, in order to generate virus-resistant transgenic plants. No noticeable adverse effects on the growth and reproduction of the transgenic plants were observed. Our results demonstrated that targeting viral DNA is an effective strategy for protecting plants from ssDNA viruses. -
dc.description.uri 1 -
dc.language English -
dc.publisher SPRINGER -
dc.subject PATHOGEN-DERIVED RESISTANCE -
dc.subject DEPENDENT RNA-POLYMERASE -
dc.subject LEAF CURL GEMINIVIRUS -
dc.subject SINGLE-CHAIN FV -
dc.subject TRANSGENIC PLANTS -
dc.subject NICOTIANA-BENTHAMIANA -
dc.subject VIRUS-RESISTANCE -
dc.subject PROTEIN -
dc.subject GENE -
dc.subject DNA -
dc.title A nucleic acid hydrolyzing recombinant antibody confers resistance to curtovirus infection in tobacco -
dc.type Article -
dc.citation.endPage 187 -
dc.citation.startPage 179 -
dc.citation.title PLANT CELL TISSUE AND ORGAN CULTURE -
dc.citation.volume 115 -
dc.citation.number 2 -
dc.identifier.bibliographicCitation PLANT CELL TISSUE AND ORGAN CULTURE, v.115, no.2, pp.179 - 187 -
dc.identifier.doi 10.1007/s11240-013-0357-4 -
dc.identifier.scopusid 2-s2.0-84885952369 -
dc.identifier.wosid 000325849300007 -
dc.type.docType Article -
dc.description.journalClass 1 -
dc.subject.keywordPlus PATHOGEN-DERIVED RESISTANCE -
dc.subject.keywordPlus DEPENDENT RNA-POLYMERASE -
dc.subject.keywordPlus LEAF CURL GEMINIVIRUS -
dc.subject.keywordPlus SINGLE-CHAIN FV -
dc.subject.keywordPlus TRANSGENIC PLANTS -
dc.subject.keywordPlus NICOTIANA-BENTHAMIANA -
dc.subject.keywordPlus VIRUS-RESISTANCE -
dc.subject.keywordPlus PROTEIN -
dc.subject.keywordPlus GENE -
dc.subject.keywordPlus DNA -
dc.subject.keywordAuthor Antibody -
dc.subject.keywordAuthor 3D8 scFv -
dc.subject.keywordAuthor DNase -
dc.subject.keywordAuthor Geminivirus -
dc.subject.keywordAuthor Nicotiana tabacum -
dc.relation.journalWebOfScienceCategory Biotechnology & Applied Microbiology -
dc.relation.journalWebOfScienceCategory Plant Sciences -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.relation.journalResearchArea Biotechnology & Applied Microbiology -
dc.relation.journalResearchArea Plant Sciences -
Appears in Collections:
South Sea Research Institute > Risk Assessment Research Center > 1. Journal Articles
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