북극 해양metagenome유래 신규 esterase들의 특성분석 및 응용

Title
북극 해양metagenome유래 신규 esterase들의 특성분석 및 응용
Alternative Title
Characterization and Its Potential Application of Two Novel Esterases Derived from the Arctic Ocean Sediment Metagenome
Author(s)
김준태; 전정호; 강성균; 이정현; 김상진
KIOST Author(s)
Kang, Sung Gyun(강성균)Lee, Jung Hyun(이정현)
Publication Year
2007-05-10
Abstract
Metagenome-based approaches are a powerful tool for exploring novel biotechnological applications from uncultured microbial communities in many natural environments. In this study, two novel esterase genes were cloned by activity-based screening a fosmid library of Arctic Ocean sediment. The sequence analysis revealed that these genes encode a protein of 303 and 312 amino acids, respectively, and share 40-50% amino acid sequence identity with members of the HSL family. The recombinant esterases were overexpressed in a soluble form in E. coli through coexpression with chaperones and purified to homogeneity. The optimum activity of the esterases occurred at 35 oC and pH 8.0, respectively. Both esterases preferentially hydrolyzed p-nitrophenyl esters of fatty acids with short chain lengths of £ 10 carbon atoms among the p-nitrophenyl esters (C2 to C18) tested. Interestingly enough, these esterases exhibited hydrolyzing activity toward racemic ofloxacin esters, indicating that it might be effectively used as a biocatalyst in the pharmaceutical industry.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/30588
Bibliographic Citation
2007국제미생물학회, pp.1, 2007
Publisher
한국미생물학회
Type
Conference
Language
English
Publisher
한국미생물학회
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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