Characterization and complete genome sequence of the Shigella bacteriophage pSf-1 SCIE SCOPUS

Cited 26 time in WEB OF SCIENCE Cited 28 time in Scopus
Title
Characterization and complete genome sequence of the Shigella bacteriophage pSf-1
Author(s)
Jun, Jin Woo; Kim, Ji Hyung; Shin, Sang Phil; Han, Jee Bun; Chai, Ji Young; Park, Se Chang
Publication Year
2013-12
Abstract
Shigellosis is a global health problem, and Shigella flexneri is the major cause of this disease. In this study, we isolated a virulent Siphoviridae bacteriophage (phage), pSf-1, that infects S. flexneri. This phage was isolated from the Han River in Korea and was found to infect S. flexneri, Shigella boydii, and Shigella sonnei. One-step growth analysis revealed that this phage has a short latent period (10 mm) and a large burst size (86.86 PFU/cell), indicating that pSf-1 has good host infectivity and effective lytic activity. The double-stranded DNA genome of pSf-1 is composed of 51,821 bp with a G C content of 44.02%. The genome encodes 94 putative ORFs, 71 putative promoters, and 60 transcriptional terminator regions. Genome sequence analysis of pSf-1 and comparative analysis with the homologous Shigella phage Shfl1 revealed that there is a high degree of similarity between pSf-1 and Shfl1 in 54 of the 94 ORFs of pSf-1. The results of this investigation indicate that pSf-1 is a novel Shigella phage and that this phage might have potential uses against shigellosis. (C) 2013 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
ISSN
0923-2508
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3050
DOI
10.1016/j.resmic.2013.08.007
Bibliographic Citation
RESEARCH IN MICROBIOLOGY, v.164, no.10, pp.979 - 986, 2013
Publisher
ELSEVIER SCIENCE BV
Subject
SONNEI-GASTROENTERITIS; KLEBSIELLA-PNEUMONIAE; ESCHERICHIA-COLI; PHAGE THERAPY; FOOD SAFETY; HOST-RANGE; OUTBREAK; FLEXNERI; INFECTION; CONSUMPTION
Keywords
Shigellosis; Shigella flexneri; Siphoviridae; Bacteriophage; pSf-1
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER SCIENCE BV
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