Characterization and complete genome sequence of the Shigella bacteriophage pSf-1 SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Jun, Jin Woo | - |
dc.contributor.author | Kim, Ji Hyung | - |
dc.contributor.author | Shin, Sang Phil | - |
dc.contributor.author | Han, Jee Bun | - |
dc.contributor.author | Chai, Ji Young | - |
dc.contributor.author | Park, Se Chang | - |
dc.date.accessioned | 2020-04-20T05:25:16Z | - |
dc.date.available | 2020-04-20T05:25:16Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2013-12 | - |
dc.identifier.issn | 0923-2508 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/3050 | - |
dc.description.abstract | Shigellosis is a global health problem, and Shigella flexneri is the major cause of this disease. In this study, we isolated a virulent Siphoviridae bacteriophage (phage), pSf-1, that infects S. flexneri. This phage was isolated from the Han River in Korea and was found to infect S. flexneri, Shigella boydii, and Shigella sonnei. One-step growth analysis revealed that this phage has a short latent period (10 mm) and a large burst size (86.86 PFU/cell), indicating that pSf-1 has good host infectivity and effective lytic activity. The double-stranded DNA genome of pSf-1 is composed of 51,821 bp with a G C content of 44.02%. The genome encodes 94 putative ORFs, 71 putative promoters, and 60 transcriptional terminator regions. Genome sequence analysis of pSf-1 and comparative analysis with the homologous Shigella phage Shfl1 revealed that there is a high degree of similarity between pSf-1 and Shfl1 in 54 of the 94 ORFs of pSf-1. The results of this investigation indicate that pSf-1 is a novel Shigella phage and that this phage might have potential uses against shigellosis. (C) 2013 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | SONNEI-GASTROENTERITIS | - |
dc.subject | KLEBSIELLA-PNEUMONIAE | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | PHAGE THERAPY | - |
dc.subject | FOOD SAFETY | - |
dc.subject | HOST-RANGE | - |
dc.subject | OUTBREAK | - |
dc.subject | FLEXNERI | - |
dc.subject | INFECTION | - |
dc.subject | CONSUMPTION | - |
dc.title | Characterization and complete genome sequence of the Shigella bacteriophage pSf-1 | - |
dc.type | Article | - |
dc.citation.endPage | 986 | - |
dc.citation.startPage | 979 | - |
dc.citation.title | RESEARCH IN MICROBIOLOGY | - |
dc.citation.volume | 164 | - |
dc.citation.number | 10 | - |
dc.contributor.alternativeName | 김지형 | - |
dc.identifier.bibliographicCitation | RESEARCH IN MICROBIOLOGY, v.164, no.10, pp.979 - 986 | - |
dc.identifier.doi | 10.1016/j.resmic.2013.08.007 | - |
dc.identifier.scopusid | 2-s2.0-84889092016 | - |
dc.identifier.wosid | 000329379400002 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | SONNEI-GASTROENTERITIS | - |
dc.subject.keywordPlus | KLEBSIELLA-PNEUMONIAE | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | PHAGE THERAPY | - |
dc.subject.keywordPlus | FOOD SAFETY | - |
dc.subject.keywordPlus | HOST-RANGE | - |
dc.subject.keywordPlus | OUTBREAK | - |
dc.subject.keywordPlus | FLEXNERI | - |
dc.subject.keywordPlus | INFECTION | - |
dc.subject.keywordPlus | CONSUMPTION | - |
dc.subject.keywordAuthor | Shigellosis | - |
dc.subject.keywordAuthor | Shigella flexneri | - |
dc.subject.keywordAuthor | Siphoviridae | - |
dc.subject.keywordAuthor | Bacteriophage | - |
dc.subject.keywordAuthor | pSf-1 | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Microbiology | - |