초고온성 고세균 써모코커스 온누리누스 엔에이원의 게놈으로부터 신규 효소의 개발

Title
초고온성 고세균 써모코커스 온누리누스 엔에이원의 게놈으로부터 신규 효소의 개발
Alternative Title
Development of novel enzymes from the genome of the hyperthermophilic archaeon Thermococcus onnurineus NA1
Author(s)
이정현; 이현숙; 강성균; 김윤재; 배승섭; 전정호; 조요나; 김상진
KIOST Author(s)
Lee, Jung Hyun(이정현)Lee, Hyun Sook(이현숙)Kang, Sung Gyun(강성균)
Publication Year
2007-09-23
Abstract
We isolated a new hyperthermophilic archaea from a deep-sea hydrothermal vent area in the PACMANUS field, and identified as a novel strain, Thermococcus onnurineus NA1 by polyphasic classification approach. Thermococcus is ubiquitous in natural high-temperature environments, and may play a major role in the biogeochemical activity of microbial consortia within hot-water ecosystems. To obtain insight into this important genus, we have determined and annotated the complete 1,847,607-base genome of Thermococcus onnurineus NA1, followed by a comparison with the complete genomes of archaeas, especially with those of thermococcales. A total of 1978 coding DNA sequences (CDSs) have been identified, among which (1,521 CDSs) had COG hits. Two thirds of the ORFs (1331 CDSs) were annotatable, whereas the functions of 647 CDSs could not be predicted from the primary structures. The genomic analysis using various bioinformatic tools showed that the strain could possess various novel enzymes of considerable biotechnological interest such as starch-hydrolyzing enzymes, proteases, DNA modifying enzymes, and so on. By combining conventional genetic engineering and genomic research techniques, enzymes were overepressed in E. coli and are being developed for biotechnological purposes.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/30358
Bibliographic Citation
Enzyme Engineering XIX, pp.60, 2007
Publisher
Enayme Engineering Conference
Type
Conference
Language
English
Publisher
Enayme Engineering Conference
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse