초고온성 고세균 써모코커스로부터 haloacid dehalogenase
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Title
- 초고온성 고세균 써모코커스로부터 haloacid dehalogenase
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Alternative Title
- A new phosphatase of the haloacid dehalogenase superfamily from the hyperthermophilic archaeon Thermococcus onnurineus NA1
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Author(s)
- 조요나; 이현숙; 강성균; 이정현
- KIOST Author(s)
- Lee, Hyun Sook(이현숙); Kang, Sung Gyun(강성균); Lee, Jung Hyun(이정현)
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Alternative Author(s)
- 조요나; 이현숙; 강성균; 이정현
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Publication Year
- 2007-09-23
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Abstract
- As a part of genomic research of the Thermococcus onnurineus NA1, a hyperthermophilic archaeon collected from a deep-sea hydrothermal vent area, a gene annotated as a hydrolase of the haloacid dehalogenase superfamily was investigated to assign its function. The gene was cloned and expressed in Escherichia coli and its recombinant protein was purified by Co2+-IMAC-Sepharose column chromatography. The enzyme possessed phosphatase activity, which wasstrictly dependent on the presence of divalent metal ion such as Co2+, Ni2+, Mn2+, or Mg2+ at optimum pH 6.5. The enzyme showed broad substrate specificity toward phosphorylated substrates (sugars, nucleotides, pyrophosphate and amino acids). While phosphatases of HAD superfamily exhibited nucleotidase activities, this phosphatase revealed high catalytic efficiency and affinity toward canonical 5¢-nucloside tri- and diphosphatates as well as nucleotidase activity.
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/30356
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Bibliographic Citation
- Enzyme Engineering Conference 19, pp.67, 2007
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Publisher
- Enzyme Conferences International
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Type
- Conference
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Language
- English
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