초고온성 고세균 써모코커스로부터 haloacid dehalogenase

Title
초고온성 고세균 써모코커스로부터 haloacid dehalogenase
Alternative Title
A new phosphatase of the haloacid dehalogenase superfamily from the hyperthermophilic archaeon Thermococcus onnurineus NA1
Author(s)
조요나; 이현숙; 강성균; 이정현
KIOST Author(s)
Lee, Hyun Sook(이현숙)Kang, Sung Gyun(강성균)Lee, Jung Hyun(이정현)
Publication Year
2007-09-23
Abstract
As a part of genomic research of the Thermococcus onnurineus NA1, a hyperthermophilic archaeon collected from a deep-sea hydrothermal vent area, a gene annotated as a hydrolase of the haloacid dehalogenase superfamily was investigated to assign its function. The gene was cloned and expressed in Escherichia coli and its recombinant protein was purified by Co2+-IMAC-Sepharose column chromatography. The enzyme possessed phosphatase activity, which wasstrictly dependent on the presence of divalent metal ion such as Co2+, Ni2+, Mn2+, or Mg2+ at optimum pH 6.5. The enzyme showed broad substrate specificity toward phosphorylated substrates (sugars, nucleotides, pyrophosphate and amino acids). While phosphatases of HAD superfamily exhibited nucleotidase activities, this phosphatase revealed high catalytic efficiency and affinity toward canonical 5¢-nucloside tri- and diphosphatates as well as nucleotidase activity.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/30356
Bibliographic Citation
Enzyme Engineering Conference 19, pp.67, 2007
Publisher
Enzyme Conferences International
Type
Conference
Language
English
Publisher
Enzyme Conferences International
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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