Symbiotic bacterial community in a marine tropical sponge sp. of Chuuk, FSM

Title
Symbiotic bacterial community in a marine tropical sponge sp. of Chuuk, FSM
Author(s)
배승섭; 전정호; 정인순; 김상진; 강성균; 권개경; 이정현
KIOST Author(s)
Kang, Sung Gyun(강성균)Kwon, Kae Kyoung(권개경)Lee, Jung Hyun(이정현)
Publication Year
2007-11-15
Abstract
To study bacterial community associated with a marine sponge sample of tropical area of Micronesia, we performed T-RFLP (terminal-restriction fragment lemgth polymorphism) analysis in comparison with that of its surrounding sea water based on 16S rDNAs gene. Also, cloning and sequencing of bacterial 16S rRNAs were carried out for the characterization of each bacterial component with terminal restriction fragments (T-RFs). Total 14 of T-RF peaks generated from a restriction enzyme, HaeIII were detected in seawater. Among them, 9 T-RFs were identified through 16S rDNA clone analysis, the which major T-RF peak (46.8% of the total clone libraries) with 291/292 base was affiliated in the Cyanobacterial phylum. Total 16 T-RFs by tetrameric restriction enzyme HaeIII, HhaI and MspI, obtained in the marine sponge tissue, were identified through 16S rDNA clone analysis. Major T-RF peaks with 68, 98 and 160 base in length (73.1% of the total clone libraries) were affiliated within r-Proteobacteria.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/30181
Bibliographic Citation
2nd SPIRITS Workshop, pp.123 - 129, 2007
Publisher
KORDI
Type
Conference
Language
English
Publisher
KORDI
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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