한국산 해양생물의 종동정을 위한 DNA 바코드 및 올리고뉴클레오티드 DNA 칩

Title
한국산 해양생물의 종동정을 위한 DNA 바코드 및 올리고뉴클레오티드 DNA 칩
Alternative Title
DNA BARCODE AND OLIGONUCLEOTIDE CHIP FOR IDENTIFICATION OF KOREAN MARINE ORGANISMS
Author(s)
김충곤; 정다금; 김고은; 최은지; 이유철; Habib; 박흥식; 배세진; 윤현규; 정진욱; 황승용; 이윤호
KIOST Author(s)
Kim, Choong Gon(김충곤)Park, Heung-Sik(박흥식)Lee, Youn Ho(이윤호)
Publication Year
2008-09-09
Abstract
We analyzed more than 250 species of COI barcode of the Korean marine organisms which are including fish, sea urchin, sea cucumber, sponge, and mollusca. The COI barcode results showed that a significant differentiation between each species using this study except the sponge species. As a rapid genetic species identification, some of these organism, we developed a DNA microarrays with oligonucleotide probes based on the DNA barcoding technology. Oligonucleotide probes have been designed from COI gene sequences. To test the usefulness of the DNA chip, fragments of the COI gene were amplified from Korean marine organisms and hybridized with the oligonucleotid probes. Distinct species specific hybridization patterns appeared on the DNA chip dependent on the species.In contrast with conventional DNA sequencing, microarray hybridizations only require one PCR and purification step, allowing faster and easier handling. These results show that DNA chip method are a feasible technology for species identification in marine organisms.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/29822
Bibliographic Citation
International Fish DNA Barcode Meeting, 2008
Publisher
FISH-BOL North East Branch
Type
Conference
Language
English
Publisher
FISH-BOL North East Branch
Related Researcher
Research Interests

Marine Molecular Genetics,Marine Ecology and Evolution,해양분자유전,해양생태및진화

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