한국산 해양생물의 종동정을 위한 DNA 바코드 및 올리고뉴클레오티드 DNA 칩

Abstract

We analyzed more than 250 species of COI barcode of the Korean marine organisms which are including fish, sea urchin, sea cucumber, sponge, and mollusca. The COI barcode results showed that a significant differentiation between each species using this study except the sponge species. As a rapid genetic species identification, some of these organism, we developed a DNA microarrays with oligonucleotide probes based on the DNA barcoding technology. Oligonucleotide probes have been designed from COI gene sequences. To test the usefulness of the DNA chip, fragments of the COI gene were amplified from Korean marine organisms and hybridized with the oligonucleotid probes. Distinct species specific hybridization patterns appeared on the DNA chip dependent on the species.In contrast with conventional DNA sequencing, microarray hybridizations only require one PCR and purification step, allowing faster and easier handling. These results show that DNA chip method are a feasible technology for species identification in marine organisms.