The Effect of Methane on The Microbial Community in The Sediment of Ulleung-Basin

Title
The Effect of Methane on The Microbial Community in The Sediment of Ulleung-Basin
Author(s)
이진우; 권개경; 박장준; 이정현
KIOST Author(s)
Kwon, Kae Kyoung(권개경)Lee, Jung Hyun(이정현)
Publication Year
2010-05-06
Abstract
Geophysical survey has shown that gas hydrate exists in deep-sea sediments at a water depth of 2,000 m in the East Sea, Korea. Bacterial community analysis in methane hydrate-bearing deep-sea sediments (ca. 8 m below the seafloor) obtained from the Ulleung-Basin was made by cultivation-independent techniques, terminal restriction fragment length polymorphism (T-RFLP) and 16S rDNA pyrosequencing. In the T-RFLP analysis, it was shown that the community structure in the possible sulfate methane transition zone (SMTZ) that was characterized as depleted sulfate and increased methane, was different from that of the upper and deeper region of the SMTZ. And then, 16S rDNA pyrosequencing analsys has been conducted to show the bacterial community structures in high-resolution. Among the total 364,000 sequences, about 180,000 reads were sorted and analysed. The homology searches revealed that the most of those sequences, more than 50% affiliated into the Chloroflexi and candidate division JS1, which were characteristic in the deep-sea marine sediments containing methane hydrate and that the proportion of sequences within δ-proteobacteria was relatively higher in the SMTZ (100 ∼ 150 cm) and surface region (0∼20 cm). This result implied that microbial community structure in the SMTZ characteristic in microbial community structure.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/28994
Bibliographic Citation
2010 한국미생물학회 국제학술대회, pp.156, 2010
Publisher
한국미생물학회
Type
Conference
Language
English
Publisher
한국미생물학회
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse