Expression, crystallization and preliminary X-ray crystallographic analysis of D-alanine-D-alanine ligase from OXA-23-producing Acinetobacter baumannii K0420859 SCIE SCOPUS

Cited 2 time in WEB OF SCIENCE Cited 2 time in Scopus
Title
Expression, crystallization and preliminary X-ray crystallographic analysis of D-alanine-D-alanine ligase from OXA-23-producing Acinetobacter baumannii K0420859
Author(s)
Kim-Hung Huynh; Huyen-Thi Tran; Tan-Viet Pham; Ho-Phuong-Thuy Ngo; Cha, Sun-Shin; Chung, Kyung Min; Lee, Sang Hee; Kang, Lin-Woo
Publication Year
2014-04
Abstract
Acinetobacter baumannii causes bacteraemia, pneumonia, other respiratory-tract and urinary-tract infections in humans. OXA-23 carbapenemase-producing A. baumannii K0420859 (A. baumannii OXA-23) is resistant to carbapenem, a common antibacterial drug. To develop an efficient and novel antibacterial drug against A. baumannii OXA-23, D-alanine-D-alanine ligase, which is essential in bacterial cell-wall synthesis, is of interest. Here, the D-alanine-D-alanine ligase (AbDdl) gene from A. baumannii OXA-23 was cloned and expressed, and the AbDdl protein was purified and crystallized; this enzyme can be used as a novel target for an antibacterial drug against A. baumannii OXA-23. The AbDdl crystal diffracted to a resolution of 2.8 angstrom and belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 113.4, b = 116.7, c = 176.5 angstrom, a corresponding V-M of 2.8 angstrom(3) Da(-1) and a solvent content of 56.3%, and six protomers in the asymmetric unit.
ISSN
1744-3091
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2884
DOI
10.1107/S2053230X14005081
Bibliographic Citation
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, v.70, pp.505 - 508, 2014
Publisher
INT UNION CRYSTALLOGRAPHY
Keywords
cell-wall synthesis; d-alanine-d-alanine ligase; OXA-23-producing Acinetobacter baumannii
Type
Article
Language
English
Document Type
Article
Publisher
INT UNION CRYSTALLOGRAPHY
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