Transcript response of soft coral (Scleronephthya gracillimum) on exposure to polycyclic aromatic hydrocarbons SCIE SCOPUS

Cited 10 time in WEB OF SCIENCE Cited 13 time in Scopus
Title
Transcript response of soft coral (Scleronephthya gracillimum) on exposure to polycyclic aromatic hydrocarbons
Author(s)
Woo, Seonock; Lee, Aekyung; Denis, Vianney; Chen, Chaolun A.; Yum, Seungshic
KIOST Author(s)
Lee, Na Yun(이나윤)Yum, Seungshic(염승식)
Publication Year
2014-01
Abstract
Polycyclic aromatic hydrocarbons (PAHs) are the most persistent organic pollutants in worldwide aquatic environments. The extensive isolation of genes responsive to PAH pollution in soft coral (Scleronephthya gracillimum) is described herein. Soft coral colonies were exposed to 100 mu g/L of a standard mixture of PAHs. Gene candidates with transcript levels that changed in response to PAH exposure were identified by differential display polymerase chain reaction (DD-PCR). There were 37 types of candidate genes identified, of which 20 were upregulated in expression and 17 were downregulated. The functions of the genes identified included oxidative stress response, ribosomal structure maintenance, molecular chaperone activity, protein kinase activation and tumorigenesis, defense mechanisms, transcription, and other biological responses. mRNA quantification was carried out using real-time quantitative PCR in eight selected genes: cytosolic malate dehydrogenase, protein disulfide isomerase, ribosomal protein L6, ral guanine nucleotide dissociation stimulator-like 1, poly(ADP-ribose) polymerase 4, peptidylglycine alpha-hydroxylating monooxygenase, a disintegrin and metalloproteinase (ADAM) metallopeptidase protein, and eukaryotic initiation factor 4 gamma 3. Changes in transcript levels were consistent with DD-PCR results. The gene candidates isolated in this study were differentially expressed and therefore have potential as molecular biomarkers for understanding coral responses to environmental stressors.
ISSN
0944-1344
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2878
DOI
10.1007/s11356-013-1958-5
Bibliographic Citation
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, v.21, no.2, pp.901 - 910, 2014
Publisher
SPRINGER HEIDELBERG
Subject
NUCLEOTIDE DISSOCIATION STIMULATOR; PROTEIN DISULFIDE-ISOMERASE; CXXC FINGER PROTEIN-1; ALLENE OXIDE SYNTHASE; CONTROL PRIMER SYSTEM; OXIDATIVE STRESS; DIFFERENTIAL DISPLAY; MOLECULAR-CLONING; MESSENGER-RNA; FACTOR FAMILY
Keywords
Polycyclic aromatic hydrocarbon stress; Differentially expressed genes; DD-PCR; qRT-PCR; Soft coral; Scleronephthya gracillimum
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER HEIDELBERG
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