Transcript response of soft coral (Scleronephthya gracillimum) on exposure to polycyclic aromatic hydrocarbons SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Woo, Seonock | - |
dc.contributor.author | Lee, Aekyung | - |
dc.contributor.author | Denis, Vianney | - |
dc.contributor.author | Chen, Chaolun A. | - |
dc.contributor.author | Yum, Seungshic | - |
dc.date.accessioned | 2020-04-20T04:58:00Z | - |
dc.date.available | 2020-04-20T04:58:00Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2014-01 | - |
dc.identifier.issn | 0944-1344 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/2878 | - |
dc.description.abstract | Polycyclic aromatic hydrocarbons (PAHs) are the most persistent organic pollutants in worldwide aquatic environments. The extensive isolation of genes responsive to PAH pollution in soft coral (Scleronephthya gracillimum) is described herein. Soft coral colonies were exposed to 100 mu g/L of a standard mixture of PAHs. Gene candidates with transcript levels that changed in response to PAH exposure were identified by differential display polymerase chain reaction (DD-PCR). There were 37 types of candidate genes identified, of which 20 were upregulated in expression and 17 were downregulated. The functions of the genes identified included oxidative stress response, ribosomal structure maintenance, molecular chaperone activity, protein kinase activation and tumorigenesis, defense mechanisms, transcription, and other biological responses. mRNA quantification was carried out using real-time quantitative PCR in eight selected genes: cytosolic malate dehydrogenase, protein disulfide isomerase, ribosomal protein L6, ral guanine nucleotide dissociation stimulator-like 1, poly(ADP-ribose) polymerase 4, peptidylglycine alpha-hydroxylating monooxygenase, a disintegrin and metalloproteinase (ADAM) metallopeptidase protein, and eukaryotic initiation factor 4 gamma 3. Changes in transcript levels were consistent with DD-PCR results. The gene candidates isolated in this study were differentially expressed and therefore have potential as molecular biomarkers for understanding coral responses to environmental stressors. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | SPRINGER HEIDELBERG | - |
dc.subject | NUCLEOTIDE DISSOCIATION STIMULATOR | - |
dc.subject | PROTEIN DISULFIDE-ISOMERASE | - |
dc.subject | CXXC FINGER PROTEIN-1 | - |
dc.subject | ALLENE OXIDE SYNTHASE | - |
dc.subject | CONTROL PRIMER SYSTEM | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | DIFFERENTIAL DISPLAY | - |
dc.subject | MOLECULAR-CLONING | - |
dc.subject | MESSENGER-RNA | - |
dc.subject | FACTOR FAMILY | - |
dc.title | Transcript response of soft coral (Scleronephthya gracillimum) on exposure to polycyclic aromatic hydrocarbons | - |
dc.type | Article | - |
dc.citation.endPage | 910 | - |
dc.citation.startPage | 901 | - |
dc.citation.title | ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH | - |
dc.citation.volume | 21 | - |
dc.citation.number | 2 | - |
dc.contributor.alternativeName | 우선옥 | - |
dc.contributor.alternativeName | 이나윤 | - |
dc.contributor.alternativeName | 염승식 | - |
dc.identifier.bibliographicCitation | ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, v.21, no.2, pp.901 - 910 | - |
dc.identifier.doi | 10.1007/s11356-013-1958-5 | - |
dc.identifier.scopusid | 2-s2.0-84891626082 | - |
dc.identifier.wosid | 000329243700009 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | NUCLEOTIDE DISSOCIATION STIMULATOR | - |
dc.subject.keywordPlus | PROTEIN DISULFIDE-ISOMERASE | - |
dc.subject.keywordPlus | CXXC FINGER PROTEIN-1 | - |
dc.subject.keywordPlus | ALLENE OXIDE SYNTHASE | - |
dc.subject.keywordPlus | CONTROL PRIMER SYSTEM | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | DIFFERENTIAL DISPLAY | - |
dc.subject.keywordPlus | MOLECULAR-CLONING | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | FACTOR FAMILY | - |
dc.subject.keywordAuthor | Polycyclic aromatic hydrocarbon stress | - |
dc.subject.keywordAuthor | Differentially expressed genes | - |
dc.subject.keywordAuthor | DD-PCR | - |
dc.subject.keywordAuthor | qRT-PCR | - |
dc.subject.keywordAuthor | Soft coral | - |
dc.subject.keywordAuthor | Scleronephthya gracillimum | - |
dc.relation.journalWebOfScienceCategory | Environmental Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Environmental Sciences & Ecology | - |