The crystal structure of Lon Protease: Molecular Architecture of Gated Entry to A Sequestered Degradation Chamber
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Title
- The crystal structure of Lon Protease: Molecular Architecture of Gated Entry to A Sequestered Degradation Chamber
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Alternative Title
- The crystal structure of hexameric Lon Protease: Molecular Architecture of Gated Entry to A Sequestered Degradation Chamber
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Author(s)
- 안영준; 정창숙; 강성균; 차선신
- KIOST Author(s)
- An, Young Jun(안영준); Kang, Sung Gyun(강성균)
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Alternative Author(s)
- 안영준; 정창숙; 강성균; 차선신
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Publication Year
- 2011-06-13
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Abstract
- The ATP-dependent Lon protease has orthologs distributed in all kingdoms of life and is required for survival when cells are subjected to various stress conditions. Lon is a tandem fusion of a molecular chaperone belonging to the AAA+ family and a protease with a serine-lysine catalytic dyad. Here, we report the 2.0 Å resolution crystal structure of the Thermococcus onnurineus NA1 Lon (TonLon). The structure is a three-tiered hexagonal cylinder with a sequestered internal proteolytic chamber accessible through a restricted channel through the AAA+ domains. Conserved axial loops together with an insertion domain containing the membrane anchor comprise a domain positioned on the apical surface of the AAA+ ring that serves as a gate to regulate substrate access to the internal unfolding and degradation chambers. Alternating AAA+ domains in the hexameric ring exist in tight- and weak-binding nucleotide states, displaying different domain orientations and intersubunit contacts, reflective of the ATP hydrolysis-coupled motions driving protein unfolding and translocation. The bowl-shaped proteolytic chamber is contiguous with the chamber formed by the AAA+ domains, and consequently internalized proteins can directly access the proteolytic sites with no further gating restrictions. The structure suggests a model by which Lon can degrade unfolded proteins and small folded proteins or prot
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/28302
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Bibliographic Citation
- 2011 international meeting of the microbiological society of korea, 2011
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Publisher
- 대한미생물학회
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Type
- Conference
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Language
- English
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