Quantification of eggs and sperm in the Black-lip pearl oyster Pinctada margaritifera using an enzyme-linked immunosorbent assay (ELISA) SCIE SCOPUS

Cited 4 time in WEB OF SCIENCE Cited 5 time in Scopus
Title
Quantification of eggs and sperm in the Black-lip pearl oyster Pinctada margaritifera using an enzyme-linked immunosorbent assay (ELISA)
Author(s)
Jeung, Hee-Do; Kang, Do-Hyung; Park, Heung-Sik; Le Moullac, Gilles; Choi, Kwang-Sik
KIOST Author(s)
Kang, Do-Hyung(강도형)Park, Heung-Sik(박흥식)
Publication Year
2014-05
Abstract
We have developed immunological probes to quantify eggs and sperm of the Black-lip pearl (BLP) oyster Pinctada margaritifera. The western blot assay revealed that the polyclonal antibodies developed in this study specifically recognized only egg and sperm proteins. These polyclonal antibodies also showed high sensitivities to the antigens, detecting 0.31-10 mu g/ml of the egg or sperm proteins in an indirect enzyme-linked immunosorbent assay (ELISA). Accordingly, we used an indirect ELISA to quantify the eggs and sperm in BLP oysters collected in December 2009 from Weno Island in Micronesia and in May 2010 from Tahiti. The gonad somatic index (GSI), a ratio of gonad weight to somatic tissue weight, of the females collected from Weno Island ranged from 3.6 to 18.4 %, while the GSI of the females collected from Tahiti ranged from 5.6 to 12.6 %. Similarly, the GSI of the male BLP oysters from Weno Island ranged from 0.8 to 8.5 %, while the GSI of the males from Tahiti ranged from 4.8 to 7.5 %. These results lead to the conclusion that the immunological probes developed in this study can be successfully applied to quantify BLP oyster gonadal tissues and that these probes can be used in studies of BLP oyster reproductive ecology based on their sensitivity, rapidity, and affordability.
ISSN
0919-9268
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2822
DOI
10.1007/s12562-014-0706-1
Bibliographic Citation
FISHERIES SCIENCE, v.80, no.3, pp.451 - 462, 2014
Publisher
SPRINGER JAPAN KK
Subject
CLAM RUDITAPES-PHILIPPINARUM; ANNUAL REPRODUCTIVE-CYCLE; CRASSOSTREA-GIGAS; PACIFIC OYSTER; PURIFICATION; BIVALVIA; MOLLUSCA; PROTEIN; GROWTH; VITELLOGENIN
Keywords
Black-lip pearl oyster; ELISA; Pinctada margaritifera; Polyclonal antibody; Quantification of eggs and sperm
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER JAPAN KK
Related Researcher
Research Interests

Marine ecology,Marine biology,Marine living resources,해양생태,해양생물 생리,해양생물자원

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