Quantification of eggs and sperm in the Black-lip pearl oyster Pinctada margaritifera using an enzyme-linked immunosorbent assay (ELISA) SCIE SCOPUS

DC Field Value Language
dc.contributor.author Jeung, Hee-Do -
dc.contributor.author Kang, Do-Hyung -
dc.contributor.author Park, Heung-Sik -
dc.contributor.author Le Moullac, Gilles -
dc.contributor.author Choi, Kwang-Sik -
dc.date.accessioned 2020-04-20T04:40:44Z -
dc.date.available 2020-04-20T04:40:44Z -
dc.date.created 2020-01-28 -
dc.date.issued 2014-05 -
dc.identifier.issn 0919-9268 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/2822 -
dc.description.abstract We have developed immunological probes to quantify eggs and sperm of the Black-lip pearl (BLP) oyster Pinctada margaritifera. The western blot assay revealed that the polyclonal antibodies developed in this study specifically recognized only egg and sperm proteins. These polyclonal antibodies also showed high sensitivities to the antigens, detecting 0.31-10 mu g/ml of the egg or sperm proteins in an indirect enzyme-linked immunosorbent assay (ELISA). Accordingly, we used an indirect ELISA to quantify the eggs and sperm in BLP oysters collected in December 2009 from Weno Island in Micronesia and in May 2010 from Tahiti. The gonad somatic index (GSI), a ratio of gonad weight to somatic tissue weight, of the females collected from Weno Island ranged from 3.6 to 18.4 %, while the GSI of the females collected from Tahiti ranged from 5.6 to 12.6 %. Similarly, the GSI of the male BLP oysters from Weno Island ranged from 0.8 to 8.5 %, while the GSI of the males from Tahiti ranged from 4.8 to 7.5 %. These results lead to the conclusion that the immunological probes developed in this study can be successfully applied to quantify BLP oyster gonadal tissues and that these probes can be used in studies of BLP oyster reproductive ecology based on their sensitivity, rapidity, and affordability. -
dc.description.uri 1 -
dc.language English -
dc.publisher SPRINGER JAPAN KK -
dc.subject CLAM RUDITAPES-PHILIPPINARUM -
dc.subject ANNUAL REPRODUCTIVE-CYCLE -
dc.subject CRASSOSTREA-GIGAS -
dc.subject PACIFIC OYSTER -
dc.subject PURIFICATION -
dc.subject BIVALVIA -
dc.subject MOLLUSCA -
dc.subject PROTEIN -
dc.subject GROWTH -
dc.subject VITELLOGENIN -
dc.title Quantification of eggs and sperm in the Black-lip pearl oyster Pinctada margaritifera using an enzyme-linked immunosorbent assay (ELISA) -
dc.type Article -
dc.citation.endPage 462 -
dc.citation.startPage 451 -
dc.citation.title FISHERIES SCIENCE -
dc.citation.volume 80 -
dc.citation.number 3 -
dc.identifier.bibliographicCitation FISHERIES SCIENCE, v.80, no.3, pp.451 - 462 -
dc.identifier.doi 10.1007/s12562-014-0706-1 -
dc.identifier.scopusid 2-s2.0-84900841164 -
dc.identifier.wosid 000336367100007 -
dc.type.docType Article -
dc.description.journalClass 1 -
dc.subject.keywordPlus CLAM RUDITAPES-PHILIPPINARUM -
dc.subject.keywordPlus ANNUAL REPRODUCTIVE-CYCLE -
dc.subject.keywordPlus CRASSOSTREA-GIGAS -
dc.subject.keywordPlus PACIFIC OYSTER -
dc.subject.keywordPlus PURIFICATION -
dc.subject.keywordPlus BIVALVIA -
dc.subject.keywordPlus MOLLUSCA -
dc.subject.keywordPlus PROTEIN -
dc.subject.keywordPlus GROWTH -
dc.subject.keywordPlus VITELLOGENIN -
dc.subject.keywordAuthor Black-lip pearl oyster -
dc.subject.keywordAuthor ELISA -
dc.subject.keywordAuthor Pinctada margaritifera -
dc.subject.keywordAuthor Polyclonal antibody -
dc.subject.keywordAuthor Quantification of eggs and sperm -
dc.relation.journalWebOfScienceCategory Fisheries -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.relation.journalResearchArea Fisheries -
Appears in Collections:
Jeju Research Institute > Jeju Marine Research Center > 1. Journal Articles
Marine Resources Research Division > Marine Bio-Resources Research Unit > 1. Journal Articles
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