Development of a novel antiviral mouse model harboring a nuclease antibody against pseudorabies virus (PRV) infection
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Title
- Development of a novel antiviral mouse model harboring a nuclease antibody against pseudorabies virus (PRV) infection
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Author(s)
- 이건섭; 황진익; 이석찬; 이택견
- KIOST Author(s)
- Lee, Taek Kyun(이택견)
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Alternative Author(s)
- 이건섭; 황진익; 이택견
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Publication Year
- 2011-10-13
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Abstract
- In the previous experiments, recombinant 3D8 scFv, isolated from an autoimmune-prone MRL mice, showed antiviral effects. HeLa cell lines expressing 3D8 scFv conferred the resistance to HSV-1 and PRV by nuclease activity of 3D8 scFv and then 3D8 scFv can be utilized as an effective antiviral agent on C57BL/6 mice. In this Paper, we identified preventive effect on 3D8 scFv transgenic C57BL/6 mice against PRV. STG90 mouse (3D8 scFv transgenic mouse line 90) exhibited high mRNA expression of 3D8 scFv by qRT-PCR in muscle and brain. Wild type C57BL/6 mouse showed 0% survival rate after PRV challenging while STG90 have a 56% survival rates after PRV intramuscular infection. Preventive effect against PRV on 3D8 scFv TG mouse was verified by qRT-PCR, southern hybridization, and immunohistochemistry. These results demonstrated that 3D8 scFv transgenic C57BL/6 mice showed preventive effect by nuclease activity and will exhibit antiviral effects against DNA and RNA virus.
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/28179
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Bibliographic Citation
- 2011-한국분자세포생물학회, pp.92, 2011
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Publisher
- 한국분자세포생물학회
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Type
- Conference
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Language
- English
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