Development of a novel antiviral mouse model harboring a nuclease antibody against pseudorabies virus (PRV) infection

Abstract

In the previous experiments, recombinant 3D8 scFv, isolated from an autoimmune-prone MRL mice, showed antiviral effects. HeLa cell lines expressing 3D8 scFv conferred the resistance to HSV-1 and PRV by nuclease activity of 3D8 scFv and then 3D8 scFv can be utilized as an effective antiviral agent on C57BL/6 mice. In this Paper, we identified preventive effect on 3D8 scFv transgenic C57BL/6 mice against PRV. STG90 mouse (3D8 scFv transgenic mouse line 90) exhibited high mRNA expression of 3D8 scFv by qRT-PCR in muscle and brain. Wild type C57BL/6 mouse showed 0% survival rate after PRV challenging while STG90 have a 56% survival rates after PRV intramuscular infection. Preventive effect against PRV on 3D8 scFv TG mouse was verified by qRT-PCR, southern hybridization, and immunohistochemistry. These results demonstrated that 3D8 scFv transgenic C57BL/6 mice showed preventive effect by nuclease activity and will exhibit antiviral effects against DNA and RNA virus.