Zinc-binding to protein surface and its application to phase determination.

Title
Zinc-binding to protein surface and its application to phase determination.
Author(s)
김민규; 안영준; 정창숙; 이상민; 차선신
KIOST Author(s)
An, Young Jun(안영준)
Alternative Author(s)
김민규; 안영준; 정창숙; 이상민; 차선신
Publication Year
2012-09-21
Abstract
Zinc is a suitable metal for anomalous dispersion phasing methods. Crystal structure determination using anomalous scattering from zinc has been limited to proteins with intrinsically bound zinc(s). Herein we report that multiple zinc ions can be charged on the surface of proteins and used for structure determination of proteins with no intrinsic zinc-binding site. The discovery of facile zinc-binding to proteins without an intrinsic zinc-binding site opens a new avenue to the usage of zinc anomalous signal for structure determination.ons can be charged on the surface of proteins and used for structure determination of proteins with no intrinsic zinc-binding site. The discovery of facile zinc-binding to proteins without an intrinsic zinc-binding site opens a new avenue to the usage of zinc anomalous signal for structure determination.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/27569
Bibliographic Citation
2012 Structure Festival, pp.38, 2012
Publisher
Korean
Type
Conference
Language
English
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