Energy conservation by oxidation of formate to carbon dioxide and hydrogen via a sodium ion current in a hyperthermophilic archaeon SCIE SCOPUS

Cited 43 time in WEB OF SCIENCE Cited 44 time in Scopus
Title
Energy conservation by oxidation of formate to carbon dioxide and hydrogen via a sodium ion current in a hyperthermophilic archaeon
Author(s)
Lim, Jae Kyu; Mayer, Florian; Kang, Sung Gyun; Mueller, Volker
KIOST Author(s)
Kang, Sung Gyun(강성균)
Publication Year
2014-08-05
Abstract
Thermococcus onnurineus NA1 is known to grow by the anaerobic oxidation of formate to CO2 and H-2, a reaction that operates near thermodynamic equilibrium. Here we demonstrate that this reaction is coupled to ATP synthesis by a transmembrane ion current. Formate oxidation leads to H+ translocation across the cytoplasmic membrane that then drives Na+ translocation. The ion-translocating electron transfer system is rather simple, consisting of only a formate dehydrogenase module, a membrane-bound hydrogenase module, and a multisubunit Na+/H+ antiporter module. The electrochemical Na+ gradient established then drives ATP synthesis. These data give a mechanistic explanation for chemiosmotic energy conservation coupled to formate oxidation to CO2 and H-2. Because it is discussed that the membrane-bound hydrogenase with the Na+/H+ antiporter module are ancestors of complex I of mitochondrial and bacterial electron transport these data also shed light on the evolution of ion transport in complex I-like electron transport chains.
ISSN
0027-8424
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2749
DOI
10.1073/pnas.1407056111
Bibliographic Citation
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.111, no.31, pp.11497 - 11502, 2014
Publisher
NATL ACAD SCIENCES
Subject
RESPIRATORY COMPLEX-I; CONVERTING NIFE HYDROGENASES; THERMOCOCCUS-ONNURINEUS NA1; SEA HYDROTHERMAL VENT; H-2 PRODUCTION; METHANOGENIC ARCHAEA; METHANOSARCINA-MAZEI; PYROCOCCUS-FURIOSUS; ANAEROBIC-BACTERIA; MODULAR EVOLUTION
Keywords
ATP synthase; proton potential; sodium ion potential; bioenergetics
Type
Article
Language
English
Document Type
Article
Publisher
NATL ACAD SCIENCES
Related Researcher
Research Interests

marine biotechnology,microbiology,해양생명공학,미생물학

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