Oligo-microarray analysis and identification of stress-immune response genes from manila clam (Ruditapes philippinarum) exposure to heat and cold stresses SCIE SCOPUS

Cited 9 time in WEB OF SCIENCE Cited 9 time in Scopus
Title
Oligo-microarray analysis and identification of stress-immune response genes from manila clam (Ruditapes philippinarum) exposure to heat and cold stresses
Author(s)
Menike, Udeni; Lee, Youngdeuk; Oh, Chulhong; Wickramaarachchi, W. D. N.; Premachandra, H. K. A.; Park, Se Chang; Lee, Jehee; De Zoysa, Mahanama
KIOST Author(s)
Lee, Young Deuk(이영득)Oh, Chul Hong(오철홍)
Publication Year
2014-10
Abstract
Thermal stress regulates the complex system of gene expression and downstream biochemical and physiological responses in aquatic species. To identify genes involved in heat stress responses in manila clam (Ruditapes philippinarum), microarray analysis was conducted using clam transcriptome generated by pyrosequencing of cDNA library. Manila clams were exposed to heat (30 +/- A 1 A degrees C) and cold (4 +/- A 1 A degrees C) stresses and compared with control animals (18 +/- A 1 A degrees C). Heat stressed animals have changed greater number of transcripts (8,306) than cold stress (7,573). Results of both heat and cold exposure has shown that over 2-fold up-regulated or down regulated (> 2-or < 2-fold) transcripts were higher at 24 h than at 6 h. It suggests that silent and constitutive express genes can activate at critical stage of thermal stress which could be between 6 and 24 h post stresses. We identified wide range of stress-immune response genes such as transcription factors, heat shock proteins, antioxidant and detoxification enzymes, inflammatory and apoptosis related genes, cell adhesion molecules, cytokines, and IFN regulatory proteins. Histological results revealed that non-specific cellular alterations such as lesions, hypertrophy, and necrosis in stressed gills could be due to decrease of gas exchange rate which may cause hypoxia.
ISSN
0301-4851
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2716
DOI
10.1007/s11033-014-3529-3
Bibliographic Citation
MOLECULAR BIOLOGY REPORTS, v.41, no.10, pp.6457 - 6473, 2014
Publisher
SPRINGER
Subject
OYSTER CRASSOSTREA-GIGAS; SUMMER MORTALITY; MYTILUS-EDULIS; BLUE MUSSELS; EXPRESSION; SHOCK; SUSCEPTIBILITY; CYTOSKELETON; TEMPERATURE; RECEPTORS
Keywords
Manila clam (Ruditapes philippinarum); Oligo-microarray; Stress-immune genes; Thermal stress; Transcriptome
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER
Related Researcher
Research Interests

Marine Biotechnology,Marine Organism Molecular genetics,Marine Bacterial engineering,해양생물공학,해양생물분자유전학,해양미생물공학

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