Oligo-microarray analysis and identification of stress-immune response genes from manila clam (Ruditapes philippinarum) exposure to heat and cold stresses SCIE SCOPUS

DC Field Value Language
dc.contributor.author Menike, Udeni -
dc.contributor.author Lee, Youngdeuk -
dc.contributor.author Oh, Chulhong -
dc.contributor.author Wickramaarachchi, W. D. N. -
dc.contributor.author Premachandra, H. K. A. -
dc.contributor.author Park, Se Chang -
dc.contributor.author Lee, Jehee -
dc.contributor.author De Zoysa, Mahanama -
dc.date.accessioned 2020-04-20T04:25:38Z -
dc.date.available 2020-04-20T04:25:38Z -
dc.date.created 2020-01-28 -
dc.date.issued 2014-10 -
dc.identifier.issn 0301-4851 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/2716 -
dc.description.abstract Thermal stress regulates the complex system of gene expression and downstream biochemical and physiological responses in aquatic species. To identify genes involved in heat stress responses in manila clam (Ruditapes philippinarum), microarray analysis was conducted using clam transcriptome generated by pyrosequencing of cDNA library. Manila clams were exposed to heat (30 +/- A 1 A degrees C) and cold (4 +/- A 1 A degrees C) stresses and compared with control animals (18 +/- A 1 A degrees C). Heat stressed animals have changed greater number of transcripts (8,306) than cold stress (7,573). Results of both heat and cold exposure has shown that over 2-fold up-regulated or down regulated (> 2-or < 2-fold) transcripts were higher at 24 h than at 6 h. It suggests that silent and constitutive express genes can activate at critical stage of thermal stress which could be between 6 and 24 h post stresses. We identified wide range of stress-immune response genes such as transcription factors, heat shock proteins, antioxidant and detoxification enzymes, inflammatory and apoptosis related genes, cell adhesion molecules, cytokines, and IFN regulatory proteins. Histological results revealed that non-specific cellular alterations such as lesions, hypertrophy, and necrosis in stressed gills could be due to decrease of gas exchange rate which may cause hypoxia. -
dc.description.uri 1 -
dc.language English -
dc.publisher SPRINGER -
dc.subject OYSTER CRASSOSTREA-GIGAS -
dc.subject SUMMER MORTALITY -
dc.subject MYTILUS-EDULIS -
dc.subject BLUE MUSSELS -
dc.subject EXPRESSION -
dc.subject SHOCK -
dc.subject SUSCEPTIBILITY -
dc.subject CYTOSKELETON -
dc.subject TEMPERATURE -
dc.subject RECEPTORS -
dc.title Oligo-microarray analysis and identification of stress-immune response genes from manila clam (Ruditapes philippinarum) exposure to heat and cold stresses -
dc.type Article -
dc.citation.endPage 6473 -
dc.citation.startPage 6457 -
dc.citation.title MOLECULAR BIOLOGY REPORTS -
dc.citation.volume 41 -
dc.citation.number 10 -
dc.contributor.alternativeName 이영득 -
dc.contributor.alternativeName 오철홍 -
dc.identifier.bibliographicCitation MOLECULAR BIOLOGY REPORTS, v.41, no.10, pp.6457 - 6473 -
dc.identifier.doi 10.1007/s11033-014-3529-3 -
dc.identifier.scopusid 2-s2.0-84918809218 -
dc.identifier.wosid 000342440000017 -
dc.type.docType Article -
dc.description.journalClass 1 -
dc.subject.keywordPlus OYSTER CRASSOSTREA-GIGAS -
dc.subject.keywordPlus SUMMER MORTALITY -
dc.subject.keywordPlus MYTILUS-EDULIS -
dc.subject.keywordPlus BLUE MUSSELS -
dc.subject.keywordPlus EXPRESSION -
dc.subject.keywordPlus SHOCK -
dc.subject.keywordPlus SUSCEPTIBILITY -
dc.subject.keywordPlus CYTOSKELETON -
dc.subject.keywordPlus TEMPERATURE -
dc.subject.keywordPlus RECEPTORS -
dc.subject.keywordAuthor Manila clam (Ruditapes philippinarum) -
dc.subject.keywordAuthor Oligo-microarray -
dc.subject.keywordAuthor Stress-immune genes -
dc.subject.keywordAuthor Thermal stress -
dc.subject.keywordAuthor Transcriptome -
dc.relation.journalWebOfScienceCategory Biochemistry & Molecular Biology -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.relation.journalResearchArea Biochemistry & Molecular Biology -
Appears in Collections:
Jeju Research Institute > Jeju Bio Research Center > 1. Journal Articles
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