A Novel Transcriptional Regulator of CO Metabolism in Thermococcus onnurineus NA1
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Title
- A Novel Transcriptional Regulator of CO Metabolism in Thermococcus onnurineus NA1
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Author(s)
- 최애란; 김민식; 이성혁; 이정현; 강성균; 이현숙
- KIOST Author(s)
- Lee, Seong Hyuk(이성혁); Lee, Jung Hyun(이정현); Kang, Sung Gyun(강성균); Lee, Hyun Sook(이현숙)
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Alternative Author(s)
- 최애란; 김민식; 이성혁; 이정현; 강성균; 이현숙
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Publication Year
- 2013-05-01
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Abstract
- The hyperthermophilic archaeon Thermococcus onnurineus NA1 is a hydrogenogenic carboxydotroph to have a gene cluster that encodes a carbon monoxide (CO)-oxidizing system. The cluster contains a gene encoding a putative regulator (rchA) which has a helix-turn-helix DNA-binding motif. RchA is phylogenetically distinguished from the known CO-dependent transcription regulators, CooA (CO oxidation activator) from Rhodospirillum rubrum or RcoM (regulator of CO metabolism) from Burkholderia xenovorans. RchA deletion mutant caused dominant growth inhibition in CO medium. RT-qPCR and western blotting showed that the levels of transcripts and proteins were not changed in the RchA deletion mutant whereas those were significantly increased in the wild type strain in the presence of CO. In this study, we present a new type of transcription regulator to regulate the expression of genes involved in the CO metabolism.which has a helix-turn-helix DNA-binding motif. RchA is phylogenetically distinguished from the known CO-dependent transcription regulators, CooA (CO oxidation activator) from Rhodospirillum rubrum or RcoM (regulator of CO metabolism) from Burkholderia xenovorans. RchA deletion mutant caused dominant growth inhibition in CO medium. RT-qPCR and western blotting showed that the levels of transcripts and proteins were not changed in the RchA deletion mutant whereas those were significantly increased in the wild type strain in the presence of CO. In this study, we present a new type of transcription regulator to regulate the expression of genes involved in the CO metabolism.
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/27104
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Bibliographic Citation
- International Meeting of the Microbiological Society of Korea, pp.276, 2013
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Publisher
- 한국미생물학회
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Type
- Conference
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Language
- English
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