석유화합물 농후배양으로부터 분리한 신종세균 Alcanivorax taeanensis

Abstract

A gram-negative rod-shaped marine bacterium, designated as MEBiC08158T was isolated from seawater collected at the Taean-province, near the Hebei Spirit tanker oil spill accident. The 16S rRNA gene sequence analysis revealed that strain MEBiC08158T showed high similarity with the members of the genus Alcanivorax (93.3~96.9 %). Growth was observed at 12.5-45.5℃, at pH 5.0-9.5 and with 0-10 % NaCl. The major isoprenoid quinone was Q-8. The dominant fatty acids were C16:0 (27.1%), C19:0 cyclo w8c (19.1%), C12:0 (13.6%), C18:1w7c (12.4%), C12:0 3OH (8.4%), and SF3 (6.1%). The strain could actively degrade relatively short linear hydrocarbons (C10~C18). Growth of the strain as well as other members of the genus Alcanivorax enhanced by supplementation of short chain organic acids. On the basis of this polyphasic taxonomic data, strain MEBiC08158T should be classified as a novel species in the genus Alcanivorax, and proposed as A. taeanensis sp. nov.